Estudi del cost de la síntesi del pèptid BP100

Abstract

Peptide chemistry was revolutionized by Robert Bruce Merrifield in 1963. The research of this biochemist focused on obtaining peptides by solid-phase synthesis (SPPS), this method stands out for its simplicity and easy production of peptides and proteins. SPPS is a fast and quite simple method, even so, it presents different aspects that are important for the synthesis, such as the selection of the resin, the solvents used, the different protecting groups, the reagents necessary to activate the -carboxyl group of the amino acid, the ninhydrin test, etc. To develop this report, extensive bibliographic research has been carried out. Some of the most relevant topics covered in this work have been: the description of the BP100 peptide, the history and development of solid-phase peptide synthesis, the steps involved in the synthesis, and, finally, the accounting for the synthesis of the BP100 peptide. The peptide studied is BP100, KKLFKKKILKYL-NH2, an antimicrobial peptide. Antimicrobial peptides (AMPs) are natural proteins found in animals, plants, insects, and bacteria. These peptides are responsible for host defense against potential pathogenic organisms. In this work, a study has been made on the accounting required to carry out the synthesis of the BP100 peptide by solid-phase synthesis. All parameters have been studied and controlled and constant monitoring has been necessary to know the costs incurred. The experimental procedure has been carried out in such a way as to minimize the cost as much as possible. Otherwise, the different properties and characteristics of the resin against different solvents have been observed and studied. Finally, the peptide has been analyzed and characterized using HPLC and MALDI-TOF techniques, which provide quantification, quantitative and qualitative analysis, and identification of the synthesized peptide. The use of these techniques has made it possible to verify and demonstrate that the peptide obtained has been correctly and successfully synthesized using the solid-phase method