Simultaneous screening of multiple substrates with an unspecific peroxygenase enabled modified alkane and alkene oxyfunctionalisations

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Knorrscheidt, Anja
orcId Soler i Parpal, Jordi​ ​ ​ ​
Soler i Parpal, Jordi
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Hünecke, Nicole
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Püllmann, Pascal
orcId Garcia Borràs, Marc​ researcherId Garcia Borràs, Marc​ scopusId Garcia Borràs, Marc​ ​
Garcia Borràs, Marc
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Weissenborn, Martin J.
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A high throughput GC-MS approach was developed, permitting the simultaneous analysis of up to three substrates and six products quantitatively from one reaction mixture. This screening approach was applied to site-saturation libraries of the novel unspecific peroxygenaseMthUPO. Using this setup enabled substantial insights from a small mutant library. Enzyme variants were identified exhibiting selective alkene epoxidation and substantially shifted regioselectivities to 2- and 1-octanol formations. Computational modelling rationalised the observed selectivity changes ​
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