Manufacture of PCL scaffolds through electrospinning technology to accommodate Triple Negative Breast Cancer cells culture
dc.contributor.author
dc.date.accessioned
2021-05-11T06:46:51Z
dc.date.available
2021-05-11T06:46:51Z
dc.date.issued
2020-01-01
dc.identifier.issn
2212-8271
dc.identifier.uri
dc.description.abstract
Two-dimensional (2D) cell culture structures are demonstrated to differ from the in vivo environment. Therefore, cells adopt a flattened morphology that may lead to a non-physiological behaviour. For instance, 2D culture induces the differentiation of the cancer stem cells (CSCs), a tumorigenic cell niche whose study is crucial in tumors with a high relapse rate such as the triple negative breast cancer (TNBC). As an alternative, electrospun scaffolds which mimic the native extracellular matrix structure have emerged as a three-dimensional culture support. In this work, two different meshes of 7.5 and 15% of poly(ε-caprolactone) (PCL) were fabricated, differentiating in their microstructure. Scaffolds exhibited similar DSC and TGA curves compared with raw PCL, indicating their purity. TNBC MDA-MB-468 cells were seeded on scaffolds and adopted a more elongated morphology when cultured on 15% PCL meshes. Hence, electrospinning PCL scaffolds may become a suitable tool to culture TNBC cells in a more physiological manner.
dc.description.sponsorship
This work was supported by Spanish grants from
Fundación Ramón Areces and Instituto de Salud Carlos III
(PI1400329). The authors are grateful to the pre-doctoral
grant (IFUdG2017/62), the support of the Catalan
Government (2017SGR00385) and RadikalSwim
(OncoSwim)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1016/j.procir.2020.05.124
dc.relation.ispartof
© Procedia CIRP, 2020, vol. 89, p. 98-103
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Articles publicats (D-EMCI)
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.uri
dc.title
Manufacture of PCL scaffolds through electrospinning technology to accommodate Triple Negative Breast Cancer cells culture
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
033093
dc.type.peerreviewed
peer-reviewed