Using Massive Parallel Sequencing for the Development, Validation, and Application of Population Genetics Markers in the Invasive Bivalve Zebra Mussel (Dreissena polymorpha)
Text Complet
Compartir
The zebra mussel (Dreissena polymorpha, Pallas, 1771) is one of the most invasive species
of freshwater bivalves, due to a combination of biological and anthropogenic factors. Once
this species has been introduced to a new area, individuals form dense aggregations that
are very difficult to remove, leading to many adverse socioeconomic and ecological consequences.
In this study, we identified, tested, and validated a new set of polymorphic microsatellite
loci (also known as SSRs, Single Sequence Repeats) using a Massive Parallel
Sequencing (MPS) platform. After several pruning steps, 93 SSRs could potentially be amplified.
Out of these SSRs, 14 were polymorphic, producing a polymorphic yield of 15.05%.
These 14 polymorphic microsatellites were fully validated in a first approximation of the genetic
population structure of D. polymorpha in the Iberian Peninsula. Based on this polymorphic
yield, we propose a criterion for establishing the number of SSRs that require
validation in similar species, depending on the final use of the markers. These results could
be used to optimize MPS approaches in the development of microsatellites as genetic
markers, which would reduce the cost of this process