{ "dc.contributor.author": "García Martínez, Tania" , "dc.contributor.author": "Vendrell i Flotats, Meritxell" , "dc.contributor.author": "Martínez Rodero, Iris" , "dc.contributor.author": "Ordóñez León, Erika Alina" , "dc.contributor.author": "Álvarez Rodríguez, Manuel" , "dc.contributor.author": "López Béjar, Manel" , "dc.contributor.author": "Yeste Oliveras, Marc" , "dc.contributor.author": "Mogas, Teresa" , "dc.date.accessioned": "2020-10-15T10:30:58Z" , "dc.date.available": "2020-10-15T10:30:59Z" , "dc.date.issued": "2020-10-13" , "dc.identifier.issn": "1422-0067" , "dc.identifier.uri": "http://hdl.handle.net/10256/18551" , "dc.description.abstract": "This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM" , "dc.format.mimetype": "application/pdf" , "dc.language.iso": "eng" , "dc.publisher": "MDPI (Multidisciplinary Digital Publishing Institute)" , "dc.relation.isformatof": "Reproducció digital del document publicat a: https://doi.org/10.3390/ijms21207547" , "dc.relation.ispartof": "International Journal of Molecular Sciences, 2020, vol. 21, núm. 20, p. 7547" , "dc.relation.ispartofseries": "Articles publicats (D-B)" , "dc.rights": "Reconeixement 4.0 Internacional" , "dc.rights.uri": "http://creativecommons.org/licenses/by/4.0" , "dc.source": "García Martínez, Tania Vendrell Flotats, Meritxell Martínez Rodero, Iris Ordóñez León, Erika Alina Álvarez Rodríguez, Manuel López Béjar, Manel Yeste Oliveras, Marc Mogas, Teresa 2020 Glutathione ethyl ester protects in vitro-maturing bovine oocytes against oxidative stress induced by subsequent vitrification/warming International Journal of Molecular Sciences 21 20 7547" , "dc.subject": "Bestiar boví -- Recursos germoplasmàtics -- Crioconservació" , "dc.subject": "Cattle -- Germplasm resources -- Cryopreservation" , "dc.subject": "Bestiar boví -- Fecunditat" , "dc.subject": "Cattle -- Fertility" , "dc.title": "Glutathione ethyl ester protects in vitro-maturing bovine oocytes against oxidative stress induced by subsequent vitrification/warming" , "dc.type": "info:eu-repo/semantics/article" , "dc.rights.accessRights": "info:eu-repo/semantics/openAccess" , "dc.type.version": "info:eu-repo/semantics/publishedVersion" , "dc.identifier.doi": "https://doi.org/10.3390/ijms21207547" , "dc.identifier.idgrec": "032087" , "dc.type.peerreviewed": "peer-reviewed" }