{ "dc.contributor.author": "Recuero, Sandra" , "dc.contributor.author": "Sánchez, José María" , "dc.contributor.author": "Mateo Otero, Yentel" , "dc.contributor.author": "Bagés-Arnal, Sandra" , "dc.contributor.author": "McDonald, Michael" , "dc.contributor.author": "Behura, Susanta K." , "dc.contributor.author": "Spencer, Thomas E." , "dc.contributor.author": "Kenny, David A." , "dc.contributor.author": "Yeste Oliveras, Marc" , "dc.contributor.author": "Lonergan, Pat" , "dc.contributor.author": "Fernández Fuertes, Beatriz" , "dc.date.accessioned": "2020-07-15T07:22:15Z" , "dc.date.available": "2020-07-15T07:22:15Z" , "dc.date.issued": "2020-07-10" , "dc.identifier.uri": "http://hdl.handle.net/10256/18305" , "dc.description": "Les dades d’expressió gènica estan disponibles públicament al repositori digital Dryad https://doi.org/10.5061/dryad.s7h44j14r i al repositori institucional DUGiDocs http://hdl.handle.net/10256/18379" , "dc.description.abstract": "An appropriate female reproductive environment is essential for pregnancy success. In several species, including mice, pigs and horses, seminal plasma (SP) components have been shown to modulate this environment, leading to increased embryo viability and implantation. Due to the characteristics of mating in the aforementioned species, SP comes into direct contact with the uterus. However, it is questionable whether any SP reaches the uterus in species that ejaculate inside the vagina, such as humans and cattle. Hence, we hypothesized that sperm, perhaps acting as a vehicle for SP factors, play a more important role in the modulation of the maternal uterine environment in these species. In addition, changes elicited by SP and/or sperm may originate in the vagina and propagate to more distal regions of the female reproductive tract. To test these hypotheses, a bovine model in which heifers were mated to intact or vasectomized bulls or were left unmated was used. RNA-sequencing of endometrial samples collected 24 h after mating with a vasectomized bull did not reveal any differentially expressed genes (DEGs) in comparison with control samples. However, the endometrium of heifers mated with intact bulls exhibited 24 DEGs when compared to heifers mated with vasectomized bulls, and 22 DEGs when compared to unmated control heifers. The expression of a set of cytokines (IL6, IL1A, IL8, and TNFA) and candidate genes identified in the endometrial RNA-sequencing (PLA2G10, CX3CL1, C4BPA, PRSS2, BLA-DQB, and CEBPD) were assessed by RT-qPCR in the vagina and oviductal ampulla. No differences in expression of these genes were observed between treatments in any region. However, mating to both intact and vasectomized bulls induced an increase in IL1A and TNFA expression in the vagina compared to the oviduct. These data indicate that sperm, but not secretions from the accessory glands alone, induce modest changes in endometrial gene expression after natural mating in cattle. However, it is not clear whether this effect is triggered by inherent sperm proteins or SP proteins bound to sperm surface at the time of ejaculation" , "dc.description.sponsorship": "This work was funded by the EU Horizon 2020 Marie Skłodowska-Curie (No. 792212) and Science Foundation Ireland (Grant No. 16/IA/4474)" , "dc.format.mimetype": "application/pdf" , "dc.language.iso": "eng" , "dc.relation.isformatof": "Reproducció digital del document publicat a: https://doi.org/10.3389/fcell.2020.00547" , "dc.relation.ispartof": "Frontiers In Cell and Developmental Biology, 2020, vol. 8, art.núm. 547" , "dc.relation.ispartofseries": "Articles publicats (D-B)" , "dc.rights": "Reconeixement 4.0 Internacional" , "dc.rights.uri": "http://creativecommons.org/licenses/by/4.0" , "dc.source": "Recuero, Sandra Sánchez, José María Mateo Otero, Yentel Bagés-Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, Thomas E. Kenny, David A. Yeste Oliveras, Marc Lonergan, Pat Fernández Fuertes, Beatriz 2020 Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model Frontiers In Cell And Developmental Biology 8 Art.núm. 547" , "dc.subject": "Bestiar boví -- Espermatozoides" , "dc.subject": "Cattle -- Spermatozoa" , "dc.subject": "Bestiar boví -- Fecunditat" , "dc.subject": "Cattle -- Fertility" , "dc.subject": "Bestiar boví -- Reproducció" , "dc.subject": "Cattle -- Reproduction" , "dc.subject": "Endometri" , "dc.subject": "Endometrium" , "dc.subject": "RNA" , "dc.title": "Mating to intact, but not vasectomized, males elicits changes in the endometrial transcriptome: insights from the bovine model" , "dc.type": "info:eu-repo/semantics/article" , "dc.rights.accessRights": "info:eu-repo/semantics/openAccess" , "dc.relation.projectID": "info:eu-repo/grantAgreement/EC/H2020/792212/EU/Maternal-paternal communication in cattle: understanding how male-induced environmental changes in the female reproductive tract influence reproductive success/M-P Communication" , "dc.type.version": "info:eu-repo/semantics/publishedVersion" , "dc.identifier.doi": "https://doi.org/10.3389/fcell.2020.00547" , "dc.identifier.idgrec": "031679" , "dc.type.peerreviewed": "peer-reviewed" , "dc.relation.dataset": "http://hdl.handle.net/10256/18379" , "dc.relation.FundingProgramme": "H2020" , "dc.relation.ProjectAcronym": "M-P Communication" , "dc.identifier.eissn": "2296-634X" }