Assessment of microbial community structure changes by amplified ribosomal DNA restriction analysis (ARDRA)
dc.contributor.author
dc.date.accessioned
2013-05-03T10:47:36Z
dc.date.available
2013-05-03T10:47:36Z
dc.date.issued
2000
dc.identifier.issn
1139-6709
dc.identifier.uri
dc.description.abstract
Amplified ribosomal DNA restriction analysis (ARDRA) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different operational conditions. The ability of ARDRA to detect these differences has been tested in modified Ludzack-Ettinger (MLE) configurations. Samples from three activated sludge wastewater treatment plants (WWTPs) with the MLE configuration were collected for both oxic and anoxic reactors, and ARDRA patterns using double enzyme digestions AluI+MspI were obtained. A matrix of Dice similarity coefficients was calculated and used to compare these restriction patterns. Differences in the community structure due to influent characteristics and temperature could be observed, but not between the oxic and anoxic reactors of each of the three MLE configurations. Other possible applications of ARDRA for detecting and monitoring changes in activated sludge systems are also discussed
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Springer Verlag Iberica
dc.relation.ispartof
International Microbiology, 2000, vol. 3, núm. 2, p. 103-106
dc.relation.ispartofseries
Articles publicats (D-EQATA)
Articles publicats (D-B)
dc.rights
Reconeixement-NoComercial-CompartirIgual 3.0 Espanya
dc.rights.uri
dc.subject
dc.title
Assessment of microbial community structure changes by amplified ribosomal DNA restriction analysis (ARDRA)
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.idgrec
004081
dc.identifier.eissn
1618-1905