Diversity of the bacterial community in the surface soil of a pear orchard based on 16S rRNA gene analysis
dc.contributor.author
dc.date.accessioned
2013-05-03T09:54:41Z
dc.date.available
2013-05-03T09:54:41Z
dc.date.issued
2010
dc.identifier.issn
1139-6709
dc.identifier.uri
dc.description.abstract
A cultivation-independent approach based on polymerase chain reaction (PCR)-amplified partial small subunit rRNA genes was used to characterize bacterial populations in the surface soil of a commercial pear orchard consisting of different pear cultivars during two consecutive growing seasons. Pyrus communis L. cvs Blanquilla, Conference, and Williams are among the most widely cultivated cultivars in Europe and account for the majority of pear production in Northeastern Spain. To assess the heterogeneity of the community structure in response to environmental variables and tree phenology, bacterial populations were examined using PCR-denaturing gradient gel electrophoresis (DGGE) followed by cluster analysis of the 16S ribosomal DNA profiles by means of the unweighted pair group method with arithmetic means. Similarity analysis of the band patterns failed to identify characteristic fingerprints associated with the pear cultivars. Both environmentally and biologically based principal-component analyses showed that the microbial communities changed significantly throughout the year depending on temperature and, to a lesser extent, on tree phenology and rainfall. Prominent DGGE bands were excised and sequenced to gain insight into the identities of the predominant bacterial populations. Most DGGE band sequences were related to bacterial phyla, such as Bacteroidetes, Cyanobacteria, Acidobacteria, Proteobacteria, Nitrospirae, and Gemmatimonadetes, previously associated with typical agronomic crop environments
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Spanish Society for Microbiology (SEM), Viguera Editores
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.2436/20.1501.01.117
dc.relation.ispartof
International Microbiology, 2010, vol. 13, núm. 3, p. 123-134
dc.relation.ispartofseries
Articles publicats (D-EQATA)
dc.rights
Attribution-NonCommercial-ShareAlike 3.0 Spain
dc.rights.uri
dc.subject
dc.title
Diversity of the bacterial community in the surface soil of a pear orchard based on 16S rRNA gene analysis
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
015443
dc.identifier.eissn
1618-1905