Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river
dc.contributor.author
dc.date.accessioned
2011-10-27T11:10:34Z
dc.date.available
2011-10-27T11:10:34Z
dc.date.issued
2010
dc.identifier.citation
Bonnineau, C., Bonet, B., Corcoll, N., i Guasch, H. (2010). Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river. Ecotoxicology, 20 (1), 293-303. Recuperat 27 octubre de 2011, a http://www.springerlink.com/content/c4372843g0854340/
dc.identifier.issn
0963-9292
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dc.description.abstract
Antioxidant enzymes are involved in important processes of cell detoxification during oxidative stress and have, therefore, been used as biomarkers in algae. Nevertheless, their limited use in fluvial biofilms may be due to the complexity of such communities. Here, a comparison between different extraction methods was performed to obtain a reliable method for catalase extraction from fluvial biofilms. Homogenization followed by glass bead disruption appeared to be the best compromise for catalase extraction. This method was then applied to a field study in a metal-polluted stream (Riou Mort, France). The most polluted sites were characterized by a catalase activity 4–6 times lower than in the low-polluted site. Results of the comparison process and its application are promising for the use of catalase activity as an early warning biomarker of toxicity using biofilms in the laboratory and in the field
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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Versió postprint del document publicat a: http://dx.doi.org/10.1007/s10646-010-0564-2
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© Ecotoxicology, 2010, vol. 20, núm. 1, p. 293-303
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Articles publicats (D-CCAA)
dc.relation.uri
dc.rights
Tots els drets reservats
dc.subject
dc.title
Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
017437
dc.identifier.eissn
1573-3017