A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
dc.contributor.author
dc.date.accessioned
2011-09-30T08:21:04Z
dc.date.available
2011-09-30T08:21:04Z
dc.date.issued
2009
dc.identifier.citation
Viñas J., i Tudela, S. (2009). A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus). PLoS ONE, 4 (10), e7606. Recuperat 30 setembre de 2011,a doi:10.1371/ journal.pone.0007606
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dc.description.abstract
Tuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation efforts on trade control. In this paper, we validated a genetic methodology that can fully distinguish between the eight Thunnus species from any kind of processed tissue.
Methodology: After testing several genetic markers, a complete discrimination of the eight tuna species was achieved using
Forensically Informative Nucleotide Sequencing based primarily on the sequence variability of the hypervariable genetic
marker mitochondrial DNA control region (mtDNA CR), followed, in some specific cases, by a second validation by a nuclear
marker rDNA first internal transcribed spacer (ITS1). This methodology was able to distinguish all tuna species, including
those belonging to the subgenus Neothunnus that are very closely related, and in consequence can not be differentiated with other genetic markers of lower variability. This methodology also took into consideration the presence of introgression
that has been reported in past studies between T. thynnus, T. orientalis and T. alalunga. Finally, we applied the methodology
to cross-check the species identity of 26 processed tuna samples. Conclusions: Using the combination of two genetic markers, one mitochondrial and another nuclear, allows a full discrimination between all eight tuna species. Unexpectedly, the genetic marker traditionally used for DNA barcoding, cytochrome oxidase 1, could not differentiate all species, thus its use as a genetic marker for tuna species identification is questioned
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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Reproducció digital del document publicat a: http://dx.doi.org/10.1371/ journal.pone.0007606
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PLoS ONE, 2009, vol.4, núm.10, p. e7606
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Articles publicats (D-B)
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Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
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dc.subject
dc.title
A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.identifier.doi
dc.identifier.idgrec
015674
dc.identifier.eissn
1932-6203