Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene
dc.contributor.author
dc.date.accessioned
2025-01-14T10:33:43Z
dc.date.available
2025-01-14T10:33:44Z
dc.date.issued
2011-08
dc.identifier.issn
1467-7644
dc.identifier.uri
dc.description.abstract
Besides the intended effects that give a genetically modified (GM) plant the desired trait, unintended differences between GM and non-GM comparable plants may also occur. Profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. Both from the food safety aspect and for research purposes, it is important to discern unintended changes produced by the transgene and its expression from those that may be attributed to other factors. Here, we show differential expression of around 0.40% transcriptome between conventional rice var. Senia and Senia-afp constitutively expressing the AFP antifungal protein. Analysis of one-fifth of the regulated sequences showed that around 35% of the unintended effects could be attributed to the process used to produce GM plants, based on in vitro tissue culture techniques. A further 15% were event specific, and their regulation was attributed to host gene disruption and genome rearrangements at the insertion site, and effects on proximal sequences. Thus, only around half the transcriptional unintended effects could be associated to the transgene itself. A significant number of changes in Senia-afp and Senia are part of the plant response to stress conditions, and around half the sequences for which up-regulation was attributed to the transgene were induced in conventional (but not transgenic) plants after wounding. Unintended effects might, as such, putatively result in widening the self-resistance characteristics because of the transgene in GM plants
dc.description.sponsorship
Aquest treball va comptar amb el suport econòmic del projecte espanyol MEC, ref. AGL2007-65903/AGR
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.relation.isformatof
Versió postprint del document publicat a: https://doi.org/10.1111/j.1467-7652.2010.00572.x
dc.relation.ispartof
© Plant Biotechnology Journal, 2011, vol. 9, núm. 6, p. 693
dc.relation.ispartofseries
Articles publicats (D-EQATA)
dc.rights
Tots els drets reservats
dc.source
Montero Mirabet, Maria Coll Rius, Anna Nadal i Matamala, Anna Messeguer i Peypoch, Joaquima Pla i de Solà-Morales, Maria 2011 Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene Plant Biotechnology Journal 9 6 693
dc.subject
dc.title
Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
013007
dc.type.peerreviewed
peer reviewed
dc.identifier.eissn
1467-7652