Response of biofilm bacterial communities to antibiotic pollutants in a Mediterranean river
dc.contributor.author
dc.date.accessioned
2024-10-04T12:55:56Z
dc.date.available
2024-10-04T12:55:56Z
dc.date.issued
2013-08-01
dc.identifier.issn
0045-6535
dc.identifier.uri
dc.description.abstract
Antibiotics are emerging contaminants, which wing to their bioactivity, may lead to short-term and long-term alterations of natural microbial communities in aquatic environment. We investigated the effects of antibiotics on biofilm bacterial communities in the Llobregat River (Northeast Spain). Three sampling sites were selected: two less polluted sites and one hotspot. River water was collected from each site and used both as inoculum and medium for growing biofilms in independent mesocosms. After 25 d of biofilm colonization, we exposed the colonized biofilms to river waters from the downstream sites (progressively contaminated by antibiotics). A control from each site was maintained where the growing biofilm was always exposed to water from the same site. The bacterial community composition, bacterial live/dead ratio and extracellular enzyme activities of the biofilms were measured before and 9 d after exposing the biofilms to increasing contaminated waters. Sixteen antibiotic compounds were detected in the water from the three sampling sites. At each site, the antibiotics present in the highest concentrations were sulfonamides, followed by quinolones and macrolides. Bacterial communities of biofilms grown with the three river waters differed markedly in their structure, but less so in terms of functional descriptors. After switching the medium water to increasing pollution, biofilms exhibited increased levels of actinobacteria (HGC), a trend that was associated to the higher antibiotic concentrations in the water. These biofilms also showed increased bacterial mortality, and decreased extracellular leucine-aminopeptidase and alkaline phosphatase. There was a significant correlation between antibiotic concentrations and biofilm responses. Our results indicate that the continuous entrance of antibiotics in running waters cause significant structural and functional changes in microbial attached communities
dc.description.sponsorship
Financial support was provided by the EU projects MODELKEY (SSPI-CT-2003-511237-2) and KEYBIOEFFECTS (MRTN-CT-2006-035695), and the Spanish projects SCARCE (Consolider-Ingenio CSD2009-00065), Flumed-Hotspots (CGL 2011-30151-C02-01) and VIECO (009/RN08/011)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsvier
dc.relation
info:eu-repo/grantAgreement/MICINN//CGL2011-30151-C02-01/ES/HOT-SPOTS BIOGEOQUIMICOS Y MICROBIANOS EN RIOS MEDITERRANEOS. ESTRUCTURA Y FUNCION DEL BIOFILM MICROBIANO Y SU IMPLICACION EN LA GESTION DE LA CALIDAD DE LAS AGUAS FLUVIALES/
info:eu-repo/grantAgreement/MICINN//CSD2009-00065/ES/Evaluación y predicción de los efectos del cambio global en la cantidad y la calidad del agua en ríos ibéricos/
dc.relation.isformatof
Versió postprint del document publicat a: https://doi.org/10.1016/j.chemosphere.2013.01.063
dc.relation.ispartof
© Chemosphere, 2013, vol. 92, núm. 9, p. 1126-1135
dc.relation.ispartofseries
Articles publicats (D-CCAA)
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.uri
dc.subject
dc.title
Response of biofilm bacterial communities to antibiotic pollutants in a Mediterranean river
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2015-08-01
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
021675
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.ProjectAcronym
dc.identifier.eissn
1879-1298