Heat Shock Protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 ºC
dc.contributor.author
dc.date.accessioned
2024-07-15T11:31:10Z
dc.date.available
2024-07-15T11:31:10Z
dc.date.issued
2024-06-11
dc.identifier.uri
dc.description.abstract
At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 °C. Six semen pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 μM), an HSP70 inhibitor, and stored at 17 °C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca2+ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function
dc.description.sponsorship
This work was supported by the Ministry of Science and Innovation, Spain (PID2020-113320RB-I00 and PRE2021-098896), the Regional Government of Catalonia (2021-SGR-00900), and the Catalan Institution for Research and Advanced Studies (ICREA)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation
PID2020-113320RB-I00
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Reproducció digital del document publicat a: https://doi.org/10.1038/s41598-024-64488-5
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Scientific Reports, 2024, vol. 14, art. núm. 13383
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Articles publicats (D-B)
dc.rights
Attribution 4.0 International (CC BY 4.0)
dc.rights.uri
dc.source
Garriga, Ferran Maside, Carolina Padilla, Lorena Recuero, Sandra Rodríguez-Gil, Joan E. Yeste Oliveras, Marc 2024 Heat Shock Protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 ºC Scientific Reports 14 Art. num. 13383
dc.subject
dc.title
Heat Shock Protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 ºC
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00/ES/IMPLICACION DE LA FUNCION MITOCONDRIAL EN LA LONGEVIDAD DEL ESPERMATOZOIDE DE ESPECIES DE INTERES PRODUCTIVO Y DISEÑO DE DILUYENTES PARA SU CONSERVACION/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
039007
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
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dc.relation.ProjectAcronym
dc.identifier.eissn
2045-2322