Evaluation and optimization of the derivatization reaction conditions of glyphosate and aminomethylphosphonic acid with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using reversed-phase chromatography
dc.contributor.author
dc.date.accessioned
2023-01-27T13:01:59Z
dc.date.available
2023-01-27T13:01:59Z
dc.date.issued
2020-10
dc.identifier.issn
1615-9306
dc.identifier.uri
dc.description.abstract
Due to the polar and ionic characteristics of glyphosate and its main metabolite, aminomethylphosphonic acid, a derivatization reaction is required before performing liquid-chromatographic determination of these compounds. In this study, reaction conditions using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as the derivatization reagent are assessed. A two-level full-factorial design is applied here to optimize the derivatization time (ranging from 0.5 to 20 min) and temperature (from 24 to 55ºC). It is found that neither of these two variables have a significant effect on the derivatization process and that the reaction is quantitatively achieved in a few seconds at room temperature (24ºC). The results obtained indicate that derivatization reaction with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate is achieved in milder conditions, with a faster kinetic reaction, than those required with the most conventional derivatization reagents used today, and the derivatives are more stable. It has been found that the most important parameter affecting the chromatographic separation is the pH of the mobile phase, as it has a significant effect on the retention time of the hydrolyzed excess of reagent. When ammonium acetate is used in the mobile phase, buffered solutions at pH around 5.0 are required
dc.description.sponsorship
Ministerio de Economía, Industria y Competitividad. Grant Number: CTM2016-78798-C02-02-P
dc.format.extent
9 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-VCH Verlag
dc.relation
CTM2016-78798-C2-2-P
dc.relation.isformatof
Versió postprint del document publicat a: https://doi.org/10.1002/jssc.202000645
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© Journal of Separation Science, 2020, vol. 43, núm. 20, p. 3931-3939
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Articles publicats (D-Q)
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Tots els drets reservats
dc.source
Sánchez Navarro, Juan Manuel Fontàs Rigau, Clàudia 2020 Evaluation and optimization of the derivatization reaction conditions of glyphosate and aminomethylphosphonic acid with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using reversed-phase chromatography Journal of Separation Science 43 20 3931 3939
dc.subject
dc.title
Evaluation and optimization of the derivatization reaction conditions of glyphosate and aminomethylphosphonic acid with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using reversed-phase chromatography
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2016-2020/CTM2016-78798-C2-2-P/ES/ DESARROLLO DE MUESTREADORES PASIVOS Y TÉCNICAS ELECTROANALÍTICAS PARA EVALUAR LA ESPECIACIÓN Y DISPONIBILIDAD DE NUTRIENTES Y CONTAMINANTES INORGÁNICOS EN AGUAS/
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
032675
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
1615-9314