Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity
dc.contributor.author
dc.date.accessioned
2023-01-25T07:04:48Z
dc.date.available
2023-01-25T07:04:48Z
dc.date.issued
2022-06-28
dc.identifier.uri
dc.description.abstract
The development of new biomarkers for human male infertility is crucial to improve the diagnosis and the prognosis of this disease. Recently, seminal microbiota was shown to be related to sperm quality parameters, suggesting an effect in human fertility and postulating it as a biomarker candidate. However, its relationship to sperm DNA integrity has not been studied yet. The aim of the present study is to characterize the seminal microbiota of a western Mediterranean population and to evaluate its relationship to sperm chromatin integrity parameters, and oxidative stress. For that purpose, 14 samples from sperm donors and 42 samples from infertile idiopathic patients were obtained and were analyzed to assess the composition of the microbiota through full-length 16S rRNA gene sequencing (Illumina MiSeq platform). Microbial diversity and relative abundances were compared to classic sperm quality parameters (macroscopic semen parameters, motility, morphology and concentration), chromatin integrity (global DNA damage, double-stranded DNA breaks and DNA protamination status) and oxidative stress levels (oxidation-reduction potential). The seminal microbiota observed of these samples belonged to the phyla Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. The most abundant genera were Finegoldia, Peptoniphilus, Anaerococcus, Campylobacter, Streptococcus, Staphylococcus, Moraxella, Prevotella, Ezakiella, Corynebacterium and Lactobacillus. To our knowledge, this is the first detection of Ezakiella genus in seminal samples. Two clusters of microbial profiles were built based on a clustering analysis, and specific genera were found with different frequencies in relation to seminal quality defects. The abundances of several bacteria negatively correlate with the sperm global DNA fragmentation, most notably Moraxella, Brevundimonas and Flavobacterium. The latter two were also associated with higher sperm motility and Brevundimonas additionally with lower oxidative-reduction potential. Actinomycetaceae, Ralstonia and Paenibacillus correlated with reduced chromatin protamination status and increased double-stranded DNA fragmentation. These effects on DNA integrity coincide in many cases with the metabolism or enzymatic activities of these genera. Significant differences between fertile and infertile men were found in the relative presence of the Propionibacteriaceae family and the Cutibacterium, Rhodopseudomonas and Oligotropha genera, which supports its possible involvement in male fertility. Our findings sustain the hypothesis that the seminal microbiome has an effect on male fertility
dc.description.sponsorship
This research was funded by the European Regional Development
Fund and Instituto de Salud Carlos III (Economy, Industry and
Competitiveness Ministry, Madrid, Spain; Project PI14/00119) and
Generalitat de Catalunya (2017SGR1796). Supported by the
European Union’s Horizon 2020 Research and Innovation scheme
under the Marie Skłodowska-Curie grant agreement No. 801342
(Tecniospring INDUSTRY; TECSPR-19-1-0003, to JR-M)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.3389/fcell.2022.937157
dc.relation.ispartof
Frontiers in Cell and Developmental Biology, 2022, vol. 10, art.núm.937157
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 International (CC BY 4.0)
dc.rights.uri
dc.source
Garcia Segura, Sergio Rey Azpiri, Javier del Closa, Laia Garcia Martínez, Iris Hobeich, Carlos Castel, Ana Belén Vidal, Francisco Benet i Català, Jordi Ribas Maynou, Jordi Oliver Bonet, Maria 2022 Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity Frontiers in Cell and Developmental Biology 10 art.núm.937157
dc.title
Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
036267
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
2296-634X