Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used
dc.contributor.author
dc.date.accessioned
2023-01-16T09:06:40Z
dc.date.available
2023-01-16T09:06:40Z
dc.date.issued
2022-12-20
dc.identifier.issn
0716-9760
dc.identifier.uri
dc.description.abstract
Based on the inconsistent literature published thus far involving infertile patients, whether intracytoplasmic sperm injection (ICSI) allows overcoming total fertilization failure due to sperm DNA fragmentation is still unclear. Related to this, female factors, which may have a significant impact on assisted reproduction outcomes, can mask male infertility. In this scenario, evaluating ICSI outcomes following cycles using healthy donor gametes could shed light on this realm, as it would avoid the influence of (un)known confounding factors present in infertile individuals. The present work, therefore, aimed to address whether single- and double-stranded sperm DNA fragmentation leads to impaired ICSI outcomes in double gamete donation cycles. The study also compared these double-gamete donation cycles to cycles in which only sperm were donated and oocytes were obtained from infertile patients. Two cohorts were included: a) the Donor-Donor (DD) cohort, which included 27 semen donor samples used in 49 ICSI cycles with young healthy oocyte donors; and b) the Donor-Infertile (DI) cohort, which involved 34 semen donor samples used in 57 ICSI cycles with oocytes from patients. Single- and double-stranded sperm DNA breaks were determined with alkaline and neutral Comet assays, respectively; ICSI was conducted following standard protocols and embryos were monitored through time-lapse microscopy. In the DD cohort, the percentage of sperm with high overall DNA damage correlated with fertilization rates (Rs = -0.666; P <0.001) and with the percentage of blastocysts per injected oocyte (Rs = -0.414; P = 0.040). In addition, sperm DNA damage delayed the first embryo division (Rs = 0.421; P = 0.036), and development from the 8-cell to the morula stage (Rs = 0.424; P = 0.034). In contrast, double-stranded DNA breaks had no effect in this cohort. As far as the DI cohort is concerned, while overall sperm DNA damage was not found to be correlated to fertilization or blastocyst rates, pronuclei formation following ICSI was delayed when the incidence of double-stranded DNA breaks was high (Rs = 0.485; P = 0.005). In conclusion, this study, which is the first involving double donation cycles (i.e., a donor-donor cohort), supports that sperm DNA damage has a detrimental impact on fertilization rates after ICSI, and delays embryo development. Moreover, the use of oocytes from infertile individuals is suggested to hide the male-factor effect
dc.description.sponsorship
This research was supported by the European Union’s Horizon 2020 research
and innovation scheme under the Marie Skłodowska-Curie grant agreement
No. 801342 (Tecniospring INDUSTRY; TECSPR-19–1-0003), to JRM; by the
Ministry of Science and Innovation, Spain (IJC2019-039615-I, to AS-H); by the
Catalan Agency for Management of University and Research Grants, Regional
Government of Catalonia, Spain (2017-SGR-1229); by the Catalan Institution
for Research and Advanced Studies (ICREA); and by La Marató de TV3 Founda‑
tion (214/857-202039)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central (BMC)
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1186/s40659-022-00409-y
dc.relation.ispartof
Biological Research, 2022, vol. 55, núm. 1, p. 41
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Ribas Maynou, Jordi Novo, Sergi Torres, Marc Salas-Huetos, Albert Rovira, Sergi Antich Díaz, Marta Yeste Oliveras, Marc 2022 Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used Biological Research 55 1 41
dc.subject
dc.title
Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
035892
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
0717-6287