Metabolic profiling of preovulatory follicular fluid in jennies
dc.contributor.author
dc.date.accessioned
2022-11-16T11:40:08Z
dc.date.available
2022-11-16T11:40:08Z
dc.date.issued
2022-12-31
dc.identifier.issn
0034-5288
dc.identifier.uri
dc.description.abstract
Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and τ-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These differences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species
dc.description.sponsorship
J.C. was funded by the project “Demetra” (Dipartamente di Eccellenza 2018–2022, CUP_C46C18000530001), Italian Ministry for Education, University and Research. I.M.-R. was awarded a scholarship from
the Spanish Ministry of Science, Innovation and Universities (BES-2017-
081962). I⋅Y.-O. was funded by the Secretary of Higher Education,
Science, Technology and Innovation (SENESCYT), Ecuador (Scheme:
Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-
000507-2019). The authors also acknowledge the support from the
Ministry of Science and Innovation, Spain (Grant: AGL2017-88329-R),
the Catalan Agency for Management of University and Research Grants,
Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229); and
the Catalan Institution for Research and Advanced Studies, Spain
(ICREA)
dc.format.extent
10 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
AGL2017-88329-R
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1016/j.rvsc.2022.10.026
dc.relation.ispartof
Research in Veterinary Science, 2022, vol. 153, p. 127-136
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
dc.rights.uri
dc.source
Catalán, Jaime Martínez Rodero, Iris Yáñez-Ortiz, Iván Mateo Otero, Yentel Flores Bragulat, Ana Nolis, Pau Carluccio, Augusto Yeste Oliveras, Marc Miró, Jordi 2022 Metabolic profiling of preovulatory follicular fluid in jennies Research in Veterinary Science 153 127 136
dc.subject
dc.title
Metabolic profiling of preovulatory follicular fluid in jennies
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO%2FDESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
035780
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.identifier.eissn
1532-2661