Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm
dc.contributor.author
dc.date.accessioned
2022-10-11T07:19:53Z
dc.date.available
2022-10-11T07:19:53Z
dc.date.issued
2022-09-15
dc.identifier.issn
0936-6768
dc.identifier.uri
dc.description.abstract
This study evaluated the bioenergetic map of mitochondria metabolism in cryopreserved bovine sperm. The detected oligomycin-sensitive basal respiration supported ATP production; frozen-thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP, as its titration with 1, 2, 4 and 6 μM did not stimulate the uncoupling activity on oxidative phosphorylation as highlighted by unresponsive oxygen consumption. The unusual effect on the stimulation of maximal respiration was not related to fibronectin- or PDL-coated plates used for cellular metabolism analysis. Conversely, irradiation of frozen-thawed bovine sperm with the red light improved mitochondrial parameters. In effect, the maximal respiration of red-light-stimulated sperm in PDL-coated plates was higher than the non-irradiated. In spite of this, red-light irradiation had no impact on membrane integrity and mitochondrial activity evaluated by epifluorescence microscopy
dc.description.sponsorship
Open Access Funding provided by Universita degli Studi di Bologna within the CRUI-CARE Agreement
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1111/rda.14261
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Reproduction in Domestic Animals, 2022, Online Version of Record before inclusion in an issue
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Articles publicats (D-B)
dc.rights
Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
dc.rights.uri
dc.source
Algieri, Cristina Blanco Prieto, Olga Llavanera, Marc Yeste Oliveras, Marc Spinaci, Marcella Mari, Gaetano Bucci, Diego Nesci, Salvatore 2022 Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm Reproduction in Domestic Animals
dc.subject
dc.title
Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
035676
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1439-0531