DUGiDocsGeneration of an induced pluripotent stem cell line from a healthy Caucasian male
dc.contributor.author
dc.date.accessioned
2022-10-06T11:36:03Z
dc.date.available
2022-10-06T11:36:03Z
dc.date.issued
2022-04-01
dc.identifier.issn
1873-5061
dc.identifier.uri
dc.description.abstract
The effects of genetic mutations on protein function can be studied in a physiologically relevant environment using tissue-specific cells differentiated from patient-derived induced pluripotent stem cells (iPSC). However, it is crucial to use iPSC derived from healthy individuals as control. We generated an iPS cell line from skin fibroblasts of a healthy Caucasian male by nucleofection of non-integrating episomal vectors. This cell line has normal karyotype, expresses pluripotency surface markers and pluripotency genes, and successfully differentiates into cells of the 3 germ layers. Therefore, it can be used as control for any disease of interest that is modelled using iPSC
dc.description.sponsorship
This work was supported by La Marató de TV3 [grant number 20153910], by Obra social “la Caixa”, by Centro Nacional de Investigaciones Cardiovasculares [grant number CNIC-03-2008] and by European research projects on rare diseases [grant number E-Rare-3 JTC 2016-2018/ AC15/00032]
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1016/j.scr.2022.102717
dc.relation.ispartof
Stem Cell Research, 2022, vol. 60, art.núm. 102717
dc.relation.ispartofseries
Articles publicats (D-CM)
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.uri
dc.subject
dc.title
Generation of an induced pluripotent stem cell line from a healthy Caucasian male
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
035269
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1876-7753