A Bifunctional Synthetic Peptide With Antimicrobial and Plant Elicitation Properties That Protect Tomato Plants From Bacterial and Fungal Infections
dc.contributor.author
dc.date.accessioned
2022-01-10T07:32:39Z
dc.date.available
2022-01-10T07:32:39Z
dc.date.issued
2021-10-18
dc.identifier.uri
dc.description.abstract
The hybrid peptide BP178 (KKLFKKILKYLAGPAGIGKFLHSAKKDEL-OH), derived from BP100 (KKLFKKILKYL) and magainin (1-10), and engineered for plant expression, had a strong bactericidal activity but not fungicidal. Moreover, the preventive spray of tomato plants with BP178 controlled infections by the plant pathogenic bacteria Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria, as well as the fungus Botrytis cinerea. The treatment of tomato plants with BP178 induced the expression of several genes according to microarray and RT-qPCR analysis. Upregulated genes coded for several pathogenesis-related proteins, including PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR9, PR10, and PR14, as well as transcription factors like ethylene transcription factors, WRKY, NAC and MYB, involved in the salicylic acid, jasmonic acid, and ethylene-signaling pathways. BP178 induced a similar gene expression pattern to flg15 according to RT-qPCR analysis, whereas the parent peptide BP100 did not trigger such as a strong plant defense response. It was concluded that BP178 was a bifunctional peptide protecting the plant against pathogen infection through a dual mechanism of action consisting of antimicrobial activity against bacterial pathogens and plant defense elicitation on plant host
dc.description.sponsorship
We thank the Government of the Generalitat de Catalunya (Xarxa de Referencia en Biotecnología, and Xarxa de Referència en Tecnologia dels Aliments, SGR09626, 2008SGR812, and 2014SGR697) for support. The research group is accredited by SGR 2014-697 and TECNIO net from Catalonia. We also thank the University of Girona for a grant for research improvement 2015–2018
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
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Reproducció digital del document publicat a: https://doi.org/10.3389/fpls.2021.756357
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Frontiers in Plant Science, 2021, vol. 12, art.núm.756357
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Articles publicats (D-EQATA)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
A Bifunctional Synthetic Peptide With Antimicrobial and Plant Elicitation Properties That Protect Tomato Plants From Bacterial and Fungal Infections
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
033988
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1664-462X