Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep
dc.contributor.author
dc.date.accessioned
2021-12-15T09:42:13Z
dc.date.available
2021-12-15T09:42:14Z
dc.date.issued
2022-11
dc.identifier.issn
0378-4320
dc.identifier.uri
dc.description.abstract
Sperm cryopreservation is one of the most important procedures in the development of biotechnologies for assisted reproduction. In some farm animals, the use of cryopreserved sperm has so many benefits for which relevance has become more evident in recent decades. Values for post-thaw sperm quality, however, are variable among species and within individuals of the same species. There is no standardized methodology for each of the stages of the cryopreservation procedure (andrological examination, semen collection, dilution, centrifugation, resuspension of the pellet with the freezing medium, packaging, freezing and post-thaw sperm evaluation), which also contributes to differences among studies. Cryotolerance markers of sperm and seminal plasma (SP) have been evaluated for prediction of ejaculate freezability. In addition, in previous research, there has been a focus on supplementing cryopreservation media with different substances, such as enzymatic and non-enzymatic antioxidants. In most studies, inclusion of these substances have led to improved post-thaw sperm quality and fertilizing capacity as a result of minimizing the adverse effects on sperm structure and function. Another approach is the use of different cryoprotectants. The aim with this review article is to provide an update on sperm cryopreservation in farm animals. The main detrimental effects of cryopreservation are described, including the negative repercussion on reproductive performance. Furthermore, the potential use of molecular biomarkers to predict sperm cryotolerance is discussed, as well as the addition of substances that can mitigate the harmful impact of freezing and thawing on sperm
dc.description.sponsorship
I.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme:
Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019). J.C. was funded by the National Agency for
Research and Development (ANID), Ministry of Education, Chile (Scheme: Becas Chile Doctorado en el Extranjero, PFCHA; Grant:
2017/72180128). The authors also acknowledge the support from the Regional Government of Catalonia, Spain (Grant: 2017-SGR1229)
Open Access funding provided thanks to the CRUE-CSIC agreement with Elsevier
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1016/j.anireprosci.2021.106904
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Animal Reproduction Science, 2022, vol. 246, art.núm. 106904
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Yáñez-Ortiz, Iván Catalán, Jaime Rodríguez Gil, Joan Enric Miró, Jordi Yeste Oliveras, Marc 2021 Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep Animal Reproduction Science 235 art.núm. 106904
dc.subject
dc.title
Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
034255
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1873-2232