Silencing of StRIK in potato suggests a role in periderm related to RNA processing and stress
dc.contributor.author
dc.date.accessioned
2021-10-19T11:50:51Z
dc.date.available
2021-10-19T11:50:51Z
dc.date.issued
2021-09-07
dc.identifier.uri
dc.description.abstract
Background
The periderm is a protective barrier crucial for land plant survival, but little is known about genetic factors involved in its development and regulation. Using a transcriptomic approach in the cork oak (Q. suber) periderm, we previously identified an RS2-INTERACTING KH PROTEIN (RIK) homologue of unknown function containing a K homology (KH)-domain RNA-binding protein, as a regulatory candidate gene in the periderm.
Results
To gain insight into the function of RIK in the periderm, potato (S. tuberosum) tuber periderm was used as a model: the full-length coding sequence of RIK, hereafter referred to as StRIK, was isolated, the transcript profile analyzed and gene silencing in potato performed to analyze the silencing effects on periderm anatomy and transcriptome. The StRIK transcript accumulated in all vegetative tissues studied, including periderm and other suberized tissues such as root and also in wounded tissues. Downregulation of StRIK in potato by RNA interference (StRIK-RNAi) did not show any obvious effects on tuber periderm anatomy but, unlike Wild type, transgenic plants flowered. Global transcript profiling of the StRIK-RNAi periderm did show altered expression of genes associated with RNA metabolism, stress and signaling, mirroring the biological processes found enriched within the in silico co-expression network of the Arabidopsis orthologue.
Conclusions
The ubiquitous expression of StRIK transcript, the flower associated phenotype and the differential expression of StRIK-RNAi periderm point out to a general regulatory role of StRIK in diverse plant developmental processes. The transcriptome analysis suggests that StRIK might play roles in RNA maturation and stress response in the periderm
dc.description.sponsorship
This work was supported by the Ministerio de Educación y Ciencia (AGL2006–07342: identification of the gene and obtention of the transgenic lines), the Ministerio de Innovación y Ciencia [AGL2009–13745, FPI grant to P.B.: Transcript profile of the gene in potato, selection of the transgenic lines], the Ministerio de Economía y Competitividad [AGL2012–36725; AGL2015–67495-C2–1-R: analysis of the periderm anatomy, periderm transcriptome and cell localization of the protein], Departament d’Universitats, Investigació i Societat de la Informació de Catalunya [PhD grant to M.S.], the University of Girona [PhD grant to S.F.-P] and The European Regional Development Fund (ERDF)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
BioMedCentral (BMC)
dc.relation
AGL2006-07342
AGL2009-13745
AGL2012-36725
AGL2015-67495-C2-1-R
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1186/s12870-021-03141-z
dc.relation.ispartof
BMC Plant Biology, 2021, vol. 21, art. núm. 409
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
Silencing of StRIK in potato suggests a role in periderm related to RNA processing and stress
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/MEC//AGL2006-07342/ES/GENOMICA FUNCIONAL DEL FELEMA DE ALCORNOQUE/
info:eu-repo/grantAgreement/MICINN//AGL2009-13745/ES/Genes Estructurales Y Reguladores Del Felema Y Su Posible Utilizacion Como Marcadores De Calidad Del Corcho/
info:eu-repo/grantAgreement/MINECO//AGL2012-36725/ES/NUEVAS ESTRATEGIAS TRANSCRIPTOMICAS PARA LOS GENES IMPLICADOS EN LA FORMACION DEL FELEMA/
info:eu-repo/grantAgreement/MINECO//AGL2015-67495-C2-1-R/ES/OMICAS E HIBRIDACION APLICADAS A LA MEJORA DE Q. SUBER/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
1471-2229