Direct but not indirect methods correlate the percentages of sperm with altered chromatin to the intensity of chromatin damage
dc.contributor.author
dc.date.accessioned
2021-09-08T07:29:40Z
dc.date.available
2021-09-08T07:29:40Z
dc.date.issued
2021-08-25
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dc.description.abstract
Although sperm chromatin damage, understood as damage to DNA or affectations in sperm protamination, has been proposed as a biomarker for sperm quality both in humans and livestock, the low incidence found in some animals raises concerns about its potential value. In this context, as separate methods measure different facets of chromatin damage, their comparison is of vital importance. This work aims at analyzing eight techniques assessing chromatin damage in pig sperm. With this purpose, cryopreserved sperm samples from 16 boars were evaluated through the following assays: TUNEL, TUNEL with decondensation, SCSA, alkaline and neutral sperm chromatin dispersion (SCD) tests, alkaline and neutral Comet assays, and chromomycin A3 test (CMA3). In all cases, the extent of chromatin damage and the percentage of sperm with fragmented DNA were determined. The degree of chromatin damage and the percentage of sperm with fragmented DNA were significantly correlated (P<0.05) in direct methods (TUNEL, TUNEL with decondensation, alkaline and neutral Comet) and CMA3, but not in the indirect ones (SCD and SCSA). Percentages of sperm with fragmented DNA determined by alkaline Comet were significantly (P<0.05) correlated with TUNEL following decondensation, and CMA3; those determined by neutral Comet were correlated with the percentage of High DNA Stainability (SCSA); those determined by SCSA were correlated with neutral and alkaline SCD, and those determined by neutral SCD were correlated with alkaline SCD. While, in pigs, percentages of sperm with fragmented DNA are directly related to the extent of chromatin damage when direct methods are used, this is not the case of indirect techniques. Thus, the results obtained herein differ from those reported for humans in which TUNEL, SCSA, alkaline SCD and alkaline Comet were found to be correlated. These findings may shed some light on the interpretation of these tests and provide some clues for the standardization of chromatin damage methods
dc.description.sponsorship
The authors acknowledge the support from the Ministry of
Science and Innovation, Spain (Grant No. AGL2017-88329-R),
the Regional Government of Catalonia, Spain (Grant No. 2017-
SGR-1229), La Marató de TV3 Foundation (Grant No. 214/857-
202039), and the University of Girona (Postdoc UdG-2020,
to JR-M)
dc.format.mimetype
application/pdf
dc.language.iso
eng
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Reproducció digital del document publicat a: https://doi.org/10.3389/fvets.2021.719319
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Frontiers In Veterinary Science, 2021, vol. 8, art.núm.719319
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Ribas Maynou, Jordi Llavanera, Marc Mateo Otero, Yentel Garcia Bonavila, Estela Delgado Bermúdez, Ariadna YYeste Oliveras, Marc 2021 Direct but not indirect methods correlate the percentages of sperm with altered chromatin to the intensity of chromatin damage Frontiers In Veterinary Science 8 art.núm.719319
dc.subject
dc.title
Direct but not indirect methods correlate the percentages of sperm with altered chromatin to the intensity of chromatin damage
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
033653
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
2297-1769