Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
dc.contributor.author
dc.date.accessioned
2021-05-18T12:44:35Z
dc.date.available
2021-05-18T12:44:35Z
dc.date.issued
2021-05-13
dc.identifier.uri
dc.description.abstract
While artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with frozen-thawed sperm, as a high amount of polymorphonuclear neutrophils (PMN) are observed within the donkey uterus six hours after AI. While PMN appear to select the sperm that ultimately reach the oviduct, two mechanisms, phagocytosis and NETosis, have been purported to be involved in that clearance. Remarkably, sperm interacts with PMN, but the presence of seminal plasma reduces that binding. As seminal plasma is a complex fluid made up of different molecules, including proteins, this study aimed to evaluate how different seminal plasma fractions, separated by molecular weight (<3, 3-10, 10-30, 30-50, 50-100, and >100 kDa), affect sperm-PMN binding. Sperm motility, viability, and sperm-PMN binding were evaluated after 0 h, 1 h, 2 h, 3 h, and 4 h of co-incubation at 38 °C. Two seminal plasma fractions, including 30-50 kDa or 50-100 kDa proteins, showed the highest sperm motility and viability. As viability of sperm not bound to PMN after 3 h of incubation was the highest in the presence of 30-50 and 50-100 kDa proteins, we suggest that both fractions are involved in the control of the jenny's post-breeding inflammatory response. In conclusion, this study has shown for the first time that specific fractions rather than the entire seminal plasma modulate sperm-PMN binding within the donkey uterus. As several proteins suggested to be involved in the control of post-AI endometritis have a molecular weight between 30 and 100 kDa, further studies aimed at determining the identity of these molecules and evaluating their potential effect in vivo are much warranted
dc.description.sponsorship
This research was supported by the Ministry of Science and Innovation, Spain (Grants: RYC2014–15581 and AGL2017-88329-R), and the Regional Government of Catalonia (2017-SGR-1229). J.C. was funded by the National Agency for Research and Development (ANID), Ministry of Education,
Chile (Scheme: Becas Chile Doctorado en el Extranjero, PFCHA; Grant: 2017/72180128). I.Y.-O. was
funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT),
Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.3390/ani11051388
dc.relation.ispartof
Animals, 2021, vol. 11, núm. 5, p. 1388
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Miró, Jordi Catalán, Jaime Marín, Henar Yáñez-Ortiz, Iván Yeste Oliveras, Marc 2021 Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey Animals 11 5 1388
dc.subject
dc.title
Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
033442
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
2076-2615