In Vitro maturation with Leukemia Inhibitory Factor prior to the vitrification of bovine oocytes improves their embryo developmental potential and gene expression in oocytes and embryos
dc.contributor.author
dc.date.accessioned
2020-11-20T11:33:27Z
dc.date.available
2020-11-20T11:33:27Z
dc.date.issued
2020-09-25
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dc.description.abstract
Oocyte cryopreservation has a significant impact on subsequent embryonic development. Herein, we investigated whether supplementing in vitro maturation medium with Leukemia Inhibitory Factor (LIF) prior to vitrification affects embryo development and gene expression at different embryo developmental stages. A panel of genes including maternal effect, epigenetics, apoptosis and heat stress was relatively quantified. The results show reduced cleavage rates after vitrification, regardless of the LIF treatment. Although not statistically different from control-vitrified oocytes, oocyte apoptosis and the blastocyst yield of LIF-vitrified oocytes were similar to their non-vitrified counterparts. Vitrification increased oocyte ZAR1, NPM2 and DPPA3 gene expression while its expression decreased in LIF-vitrified oocytes to similar or close levels to those of non-vitrified oocytes. With a few gene-specific exceptions, vitrification significantly increased the expression of DNMT3A, HDAC1, KAT2A, BAX and BCL2L1 in oocytes and most stages of embryo development, while comparable expression patterns for these genes were observed between LIF-vitrified and non-vitrified groups. Vitrification increased HSPA1A expression in oocytes and HSP90AA1 in 2-cell embryos. Our data suggest that vitrification triggers stage-specific changes in gene expression throughout embryonic development. However, the inclusion of LIF in the IVM medium prior to vitrification stimulates blastocyst development and several other developmental parameters and induces oocytes and embryos to demonstrate gene expression patterns similar to those derived from non-vitrified oocytes
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application/pdf
dc.language.iso
eng
dc.publisher
MDPI (Multidisciplinary Digital Publishing Institute)
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Reproducció digital del document publicat a: https://doi.org/10.3390/ijms21197067
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International Journal of Molecular Sciences, 2020, vol. 21, núm. 19, p. 7067
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
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dc.source
Vendrell-Flotats, Meritxell García Martínez, Tania Martínez Rodero, Iris López Béjar, Manel Lamarre, Jonathan Yeste Oliveras, Marc Mogas Amorós, Teresa 2020 In Vitro maturation with Leukemia Inhibitory Factor prior to the vitrification of bovine oocytes improves their embryo developmental potential and gene expression in oocytes and embryos International Journal of Molecular Sciences 21 19 7067
dc.subject
dc.title
In Vitro maturation with Leukemia Inhibitory Factor prior to the vitrification of bovine oocytes improves their embryo developmental potential and gene expression in oocytes and embryos
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
032032
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1422-0067