DUGiDocsExosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro
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dc.date.accessioned
2020-09-07T07:49:17Z
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2020-09-07T07:49:17Z
dc.date.issued
2020-08-14
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1743-5889
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dc.description.abstract
Aims: To investigate exosomes as a non-invasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-hours of exposure to exosomes, there were no significant deleterious effects with regards to sperm motility, viability, membrane integrity and mitochondrial membrane potential (P>0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility
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The authors acknowledge generous support from the Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581 and AGL2016-81890-REDT), and the Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229), the Nuffield
Department of Women’s and Reproductive Health, University of Oxford, UK, the Wellcome Trust (100262Z/12/Z), Kennedy Trust for Rheumatology Research for Imaging facilities, and European Commission (ERC-2014-AdG 670930)
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application/pdf
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eng
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Reproducció digital del document publicat a: https://doi.org/10.2217/nnm-2020-0056
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Nanomedicine, 2020, vol. 15, núm. 20
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Articles publicats (D-B)
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Reconeixement 4.0 Internacional
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dc.source
Vilanova Perez, Teresa Jones, Celine Balint, Stefan Dragovic, Rebecca Dustin, Michael L. Yeste Oliveras, Marc Coward, Kevin 2020 Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro Nanomedicine 15 20
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dc.title
Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro
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info:eu-repo/semantics/article
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info:eu-repo/semantics/openAccess
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info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/
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info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
031654
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dc.type.peerreviewed
peer-reviewed
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