Effect of exposure to seminal plasma through natural mating in cattle on conceptus length and gene expression
dc.contributor.author
dc.date.accessioned
2020-05-25T07:35:06Z
dc.date.available
2020-05-25T07:35:06Z
dc.date.issued
2020-05-12
dc.identifier.uri
dc.description.abstract
A growing body of evidence suggests that paternal factors have an impact on offspring development. These studies have been mainly carried out in mice, where seminal plasma (SP) has been shown to regulate endometrial gene expression and impact embryo development and subsequent offspring health. In cattle, infusion of SP into the uterus also induces changes in endometrial gene expression, however, evidence for an effect of SP on early embryo development is lacking. In addition, during natural mating, the bull ejaculates in the vagina; hence, it is not clear whether any SP reaches the uterus in this species. Thus, the aim of the present study was to determine whether SP exposure leads to improved early embryo survival and developmental rates in cattle. To this end, Day 7 in vitro produced blastocysts were transferred to heifers (12-15 per heifer) previously mated to vasectomized bulls (n = 13 heifers) or left unmated (n = 12 heifers; control). At Day 14, heifers were slaughtered, and conceptuses were recovered to assess size, morphology and expression of candidate genes involved in different developmental pathways. Additionally, CL volume at Day 7, and weight and volume of CL at Day 14 were recorded. No effect of SP on CL volume and weight not on conceptus recovery rate was observed. However, filamentous conceptuses recovered from SP-exposed heifers were longer in comparison to the control group and differed in expression of CALM1, CITED1, DLD, HNRNPDL, PTGS2, and TGFB3. In conclusion, data indicate that female exposure to SP during natural mating can affect conceptus development in cattle. This is probably achieved through modulation of the female reproductive environment at the time of mating
dc.description.sponsorship
This work was supported by the European Union’s Horizon
2020 Research and Innovation Program (Grant Agreement
No. 792212) and Science Foundation Ireland (Grant
No. 16/IA/4474)
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application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
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Reproducció digital del document publicat a: https://doi.org/10.3389/fcell.2020.00341
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Frontiers In Cell And Developmental Biology, 2020, vol. 8, art.núm. 341
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Articles publicats (D-B)
dc.rights
Attribution 4.0 International
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dc.source
Mateo Otero, Yentel Sánchez, José María Recuero, Sandra Bagés-Arnal, Sandra McDonald, Michael Kenny, David A. Yeste Oliveras, Marc Lonergan, Pat Fernández Fuertes, Beatriz 2020 Effect of exposure to seminal plasma through natural mating in cattle on conceptus length and gene expression Frontiers In Cell And Developmental Biology 8 art.núm. 341
dc.subject
dc.title
Effect of exposure to seminal plasma through natural mating in cattle on conceptus length and gene expression
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/EC/H2020/792212/EU/Maternal-paternal communication in cattle: understanding how male-induced environmental changes in the female reproductive tract influence reproductive success/M-P Communication
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
031476
dc.type.peerreviewed
peer-reviewed
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dc.relation.ProjectAcronym
dc.identifier.eissn
2296-634X