Cryotolerance of stallion spermatozoa relies on GLPs rather than orthodox AQPs
dc.contributor.author
dc.date.accessioned
2019-11-25T08:56:53Z
dc.date.available
2019-11-25T08:56:53Z
dc.date.issued
2019-11-12
dc.identifier.uri
dc.description.abstract
Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the use of three different inhibitors: acetazolamide (AC), phloretin (PHL) and propanediol (PDO). Sperm quality and function parameters were evaluated in the presence or absence of each inhibitor in fresh and frozen-thawed samples. In the presence of AC, different parameters were altered (p < 0.05), but not in a concentration- or time-depending manner. PHL was found to decrease sperm motility, viability, acrosome integrity, and the percentages of spermatozoa with low membrane lipid disorder, high mitochondrial membrane potential (MMP) and high intracellular levels of calcium and superoxides (p < 0.05). Finally, the sperm motility, viability, acrosome integrity, the percentages of spermatozoa with low membrane lipid disorder, high MMP and high intracellular calcium levels were higher (p < 0.05) in PDO treatments than in the control. The sperm response to AC, PHL and PDO indicates that GLPs, rather than orthodox AQPs, play a crucial role during stallion sperm cryopreservation. Furthermore, post-thaw sperm quality was higher in PDO treatments than in the control, suggesting that this molecule is a potential permeable cryoprotectant
dc.description.sponsorship
This research was funded by the Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581, AGL2017-88329-R and PRE2018-083488), and the Regional Government of Catalonia, Spain (2017-SGR-1229)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
MDPI (Multidisciplinary Digital Publishing Institute)
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Reproducció digital del document publicat a: https://doi.org/10.3390/biology8040085
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Biology, 2019, vol. 8, núm. 4, p. 85
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Delgado Bermúdez, Ariadna Noto, Federico Bonilla Correal, Sebastián Garcia Bonavila, Estela Catalán, Jaime Papas, Marion Bonet, Sergi Miró, Jordi Yeste Oliveras, Marc 2019 Cryotolerance of stallion spermatozoa relies on GLPs rather than orthodox AQPs Biology 8 4 85
dc.subject
dc.title
Cryotolerance of stallion spermatozoa relies on GLPs rather than orthodox AQPs
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
030368
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
2079-7737