Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance
dc.contributor.author
dc.date.accessioned
2019-11-25T08:27:58Z
dc.date.available
2019-11-25T08:27:58Z
dc.date.issued
2019-11-09
dc.identifier.issn
2076-3921
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dc.description.abstract
While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains the main source of antioxidant enzymes able to scavenge these reactive oxygen species. Therefore, stallion seminal plasma components may have an impact on ejaculate freezability. Against this background, this study was designed to investigate whether the activities of the main stallion seminal plasma antioxidant enzymes are related to sperm cryotolerance. With this purpose, 16 ejaculates were collected from 14 healthy stallions, and each ejaculate was split into two aliquots. The first one was used to evaluate the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) in seminal plasma. The second aliquot was extended and then processed for cryopreservation. Sperm motility and viability were evaluated before and after cryopreservation, and ejaculates were classified as of good (GFE) or poor freezability (PFE) based on total motile and viable spermatozoa at post-thaw. We observed that, while the specific activities of CAT, GPX, and GSR were similar between GFE and PFE, that of SOD was significantly (p < 0.05) higher in GFE than in PFE. We can thus conclude that, in stallions, the specific activity of SOD in the seminal plasma of a given ejaculate might be related to its freezability
dc.description.sponsorship
This research was funded by the European Commission (grant number: H2020-MSCA-IF-79212), the Ministry of Science, Innovation and Universities, Spain (grants: RYC-2014-15581 and AGL2017-88329-R), and the Regional Government of Catalonia, Spain (2017-SGR-1229)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
MDPI (Multidisciplinary Digital Publishing Institute)
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Reproducció digital del document publicat a: https://doi.org/10.3390/antiox8110539
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Antioxidants, 2019, vol. 8, núm. 11, p. 539
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
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dc.source
Papas, Marion Catalán, Jaime Fernández Fuertes, Beatriz Arroyo, Laura Bassols, Anna Miró, Jordi Yeste Oliveras, Marc 2019 Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance Antioxidants 8 11 539
dc.subject
dc.title
Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
030381
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
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dc.relation.ProjectAcronym