[NiFe]-hydrogenases are constitutively expressed in an enriched Methanobacterium sp. population during electromethanogenesis
dc.contributor.author
dc.date.accessioned
2019-11-22T11:35:08Z
dc.date.available
2019-11-22T11:35:08Z
dc.date.issued
2019-04-11
dc.identifier.uri
dc.description.abstract
Electromethanogenesis is the bioreduction of carbon dioxide (CO2) to methane (CH4) utilizing an electrode as electron donor. Some studies have reported the active participation of Methanobacterium sp. in electron capturing, although no conclusive results are available. In this study, we aimed at determining short-time changes in the expression levels of [NiFe]-hydrogenases (Eha, Ehb and Mvh), heterodisulfide reductase (Hdr), coenzyme F420-reducing [NiFe]-hydrogenase (Frh), and hydrogenase maturation protein (HypD), according to the electron flow in independently connected carbon cloth cathodes poised at– 800 mV vs. standard hydrogen electrode (SHE). Amplicon massive sequencing of cathode biofilm confirmed the presence of an enriched Methanobacterium sp. population (>70% of sequence reads), which remained in an active state (78% of cDNA reads), tagging this archaeon as the main methane producer in the system. Quantitative RT-PCR determinations of ehaB, ehbL, mvhA, hdrA, frhA, and hypD genes resulted in only slight (up to 1.5 fold) changes for four out of six genes analyzed when cells were exposed to open (disconnected) or closed (connected) electric circuit events. The presented results suggested that suspected mechanisms for electron capturing were not regulated at the transcriptional level in Methanobacterium sp. for short time exposures of the cells to connected-disconnected circuits. Additional tests are needed in order to confirm proteins that participate in electron capturing in Methanobacterium sp
dc.description.sponsorship
This work was funded by Spanish Government (MINECO, CTQ2014-53718-R) and the Universitat
de Girona (MPCUdG2016/139 and MCPUdG2016/121). Sebastià Puig is a Serra
Hunter Fellow (UdG-AG-575). Ramiro Blasco-Gómez is grateful for the Research Personnel
Training (FPI) grant (BES-2015-074229). Elisabet Perona-Vico received a Research Training
grant from the University of Girona (IFUdG2018/52). LEQUiA and IEA have been recognized
as consolidated research groups by the Generalitat de Catalunya (2017SGR-1552, and
2017SGR-548)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science (PLoS)
dc.relation
info:eu-repo/grantAgreement/MINECO//CTQ2014-53718-R/ES/TECNOLOGIAS INNOVADORAS PARA LA MEJORA DE BIOGAS: DESDE LA INVESTIGACION BASICA A LA EVALUACION DE TECNOLOGIAS/
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1371/journal.pone.0215029
dc.relation.ispartof
PLoS ONE, 2019, vol. 14, núm. 4, p. e0215029
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
[NiFe]-hydrogenases are constitutively expressed in an enriched Methanobacterium sp. population during electromethanogenesis
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
029747
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.ProjectAcronym
dc.identifier.eissn
1932-6203