Amino Acid Substitutions and Differential Gene Expression of Outer Membrane Proteins in Adherent-Invasive Escherichia coli
dc.contributor.author
dc.date.accessioned
2019-11-12T09:13:30Z
dc.date.available
2019-11-12T09:13:30Z
dc.date.issued
2019-08-06
dc.identifier.uri
dc.description.abstract
Variations in the sequence and/or the expression of outer membrane proteins (OMPs) may modulate bacterial virulence. OmpA and OmpC have been involved in the interaction of adherent-invasive Escherichia coli (AIEC) strain LF82 with intestinal epithelial cells (IECs). Scarce data exist about OMPs sequence variants in a collection of AIEC strains, and no study of OMPs expression during infection exists. We aimed to determine whether particular mutations or differential expression of OMPs are associated with AIEC virulence. The ompA, ompC, and ompF genes in 14 AIEC and 30 non-AIEC strains were sequenced by Sanger method, and the protein expression profile was analyzed by urea-SDS-PAGE. Gene expression was determined during in vitro bacterial infection of intestine-407 cells by RT-qPCR. The distribution of amino acid substitutions in OmpA-A200V, OmpC-S89N, V220I, and W231D associated with pathotype and specific changes (OmpA-A200V, OmpC-V220I, D232A, OmpF-E51V, and M60K) correlated with adhesion and/or invasion indices but no particular variants were found specific of AIEC. OMPs protein levels did not differ according to pathotype when growing in Mueller-Hinton broth. Interestingly, higher OMPs gene expression levels were reported in non-AIEC growing in association with cells compared with those non-AIEC strains growing in the supernatants of infected cultures (p < 0.028), whereas in AIEC strains ompA expression was the only increased when growing in association with cells (p = 0.032), but they did not significantly alter ompC and ompF expression under this condition (p > 0.146). Despite no particular OMPs sequence variants have been found as a common and distinctive trait in AIEC, some mutations could facilitate a better interaction with the host. Moreover, the different behavior between pathotypes regarding OMPs gene expression at different stages of infection could be related with the virulence of the strains
dc.description.sponsorship
This work was funded by the Universitat de Girona projects MPCUdG2016-009 and GdRCompetUdG2017, and the Spanish Ministry of Education and Science through projects SAF2017-82261-P and SAF2013-43284-P, being the last co-funded by the European Regional Development Fund. CC-F was a recipient of an IF grant from the Universitat de Girona (IFUDG2015/12)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.relation
SAF2013-43284-P
SAF2017-82261-P
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.3389/fmicb.2019.01707
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Frontiers in Microbiology, 2019, vol. 10, art.núm. 1707
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Articles publicats (D-B)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.title
Amino Acid Substitutions and Differential Gene Expression of Outer Membrane Proteins in Adherent-Invasive Escherichia coli
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-82261-P/ES/BUSQUEDA DE NUEVOS FACTORES IMPLICADOS EN LA VIRULENCIA DEL PATOTIPO AIEC ASOCIADO A LA ENFERMEDAD DE CROHN: ESTUDIO TRANSCRIPTOMICO Y ROL DE LAS VESICULAS DE MEMBRANA EXTERNA/
info:eu-repo/grantAgreement/MINECO//SAF2013-43284-P/ES/DETERMINANTES GENETICOS IMPLICADOS EN EL PATOTIPO ESCHERICHIA COLI ADHERENTE INVASIVA (AIEC) PARA SU IDENTIFICACION EN LA ENFERMEDAD DE CROHN/
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
030128
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
1664-302X