Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation
dc.contributor.author
dc.date.accessioned
2019-11-08T07:37:14Z
dc.date.available
2019-11-08T07:37:14Z
dc.date.issued
2019-10-21
dc.identifier.issn
2045-2322
dc.identifier.uri
dc.description.abstract
This study aimed to determine the effect of bull seminal plasma (SP) and sperm on endometrial function. Bovine endometrial explants were incubated with: ejaculated sperm with or without SP, epididymal sperm, or SP alone. Neither ejaculated nor epididymal sperm induced differential expression of IL1A, IL1B, IL6, IL8, PTGES2, TNFA, and LIF. Interestingly, SP had a detrimental effect on endometrial RNA integrity. Addition of an RNase inactivation reagent to SP blocked this effect, evidencing a role for a SP-RNase. Because bulls deposit the ejaculate in the vagina, we hypothesized that the bovine endometrium is more sensitive to SP-RNase than vaginal and cervical tissues (which come into contact with SP during mating), or to endometrium from intrauterine ejaculators (such as the horse). In addition, due to differences in SP-RNase abundance depending on SP collection method (i.e., with an artificial vagina, AV, or by electroejaculation, EE), this effect was also tested. Bull SP, collected by AV, degrades RNA of mare endometrium, and bovine vagina, cervix and endometrium. However, stallion SP or bull SP collected by EE did not elicit this effect. Thus, results do not support a role for SP in modulating endometrial function to establish pregnancy in cattle
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1038/s41598-019-51413-4
dc.relation.ispartof
Scientific Reports, 2019, vol. 9, art.núm.15072
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.relation.projectID
info:eu-repo/grantAgreement/EC/H2020/792212/EU/Maternal-paternal communication in cattle: understanding how male-induced environmental changes in the female reproductive tract influence reproductive success/M-P Communication
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
030200
dc.type.peerreviewed
peer-reviewed
dc.relation.FundingProgramme
dc.relation.ProjectAcronym