Expression patterns and genetic variation of the ovine skeletal muscle transcriptome of sheep from five Spanish meat breeds
dc.contributor.author
dc.date.accessioned
2019-09-24T08:22:55Z
dc.date.available
2019-09-24T08:22:55Z
dc.date.issued
2018-07-11
dc.identifier.issn
2045-2322
dc.identifier.uri
dc.description.abstract
The goal of the current study is to analyse the gene expression profile of the ovine skeletal muscle as well as to characterize the genetic variation of transcripts expressed in such tissue. This aim has been achieved by sequencing the longissimus dorsi transcriptomes of 50 sheep distributed in five pools representing the Canaria de Pelo, Roja Mallorquina, Gallega, Xisqueta and Ripollesa Spanish autochthonous breeds. Approximately, 363 million reads per pool have been produced and 71.9–82.9% have been successfully mapped to the ovine genome in a paired-end mode (2 × 75 bp). The 200 most expressed muscle transcripts (≈1% of the total transcript count) account for 51% (Canaria de Pelo) to 67% (Gallega) of the total ovine skeletal muscle mRNA expression. These highly expressed genes play key roles in pathways related with striated muscle contraction, gluconeogenesis, glycolysis, citric acid cycle and respiratory electron transport. RNA-Sequencing of muscle transcripts has also revealed that ~72% of the SNPs detected with this approach are shared by at least two pools, and 10% of them segregate in the five pools under analysis. Most of the substitutions detected by RNA-Seq are synonymous or missense and only a minority are predicted to have consequences on protein function
dc.description.sponsorship
This research was partially funded by a grant (RZ2011-00015-C03-01) from the Instituto Nacional de
Investigación y Tecnología Agraria y Alimentaria (Spain) and by the CERCA Programme/Generalitat de
Catalunya. We acknowledge financial support from the Spanish Ministry of Economy and Competitiveness
through the Severo Ochoa Programme for Centres of Excellence in R&D 2016-2019 (SEV-2015-0533). A.
Cánovas received a fellowship from the Juan de la Cierva program (JCI-2011-10804). Tainã F Cardoso was funded
with a fellowship from the CAPES Foundation-Coordination of Improvement of Higher Education, Ministry of
Education (MEC) of the Federal Government of Brazil. The authors are grateful to the Canaria de Pelo, Gallega
Ripollesa, Roja Mallorquina and Xisqueta breeding associations for their collaboration in sample collection
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1038/s41598-018-28760-9
dc.relation.ispartof
Scientific Reports, 2018, vol. 8, núm. art. 10486
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
Expression patterns and genetic variation of the ovine skeletal muscle transcriptome of sheep from five Spanish meat breeds
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
029689
dc.type.peerreviewed
peer-reviewed