Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake
dc.contributor.author
dc.date.accessioned
2019-09-20T07:17:53Z
dc.date.available
2019-09-20T07:17:53Z
dc.date.issued
2018-03-01
dc.identifier.issn
1613-4125
dc.identifier.uri
dc.description.abstract
We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results: EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin-stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin-stimulated 2-deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin-mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2-deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion: EVs released by stressed adipocytes impair insulin action in neighboring adipocytes
dc.description.sponsorship
J.M. and F.J.O. contributed equally to this wortk. J.M. was a joint Ph.D student between University of Liverpool and CICbioGUNE. F.J.O. was supported by the foundation Daniel Bravo. Funding: MINECO (SAF2015‐66312) to J.M.F.P., Fondo Europeo de Desarrollo Regional (FEDER) and CIBER obesidad (CIBERobn), Instituto de Salud Carlos III to J.M.F.R. J.M., F.J.O., and S.M. performed data acquisition and analysis; J.M., F.J.O., J.M.F.P., M.W., J.M.F.R., and S.M. performed writing of manuscript
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-VCH Verlag
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1002/mnfr.201700917
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Molecular Nutrition and Food Research, 2018, vol. 62, núm. 5, p. 1700917
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Articles publicats (IdIBGi)
dc.rights
Attribution 4.0 International
dc.rights.uri
dc.subject
dc.title
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
027756
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1613-4133