Estimating Detection Limits in Chromatography from Calibration Data: Ordinary Least Squares Regression vs. Weighted Least Squares
dc.contributor.author
dc.date.accessioned
2018-10-17T07:03:40Z
dc.date.available
2018-10-17T07:03:40Z
dc.date.issued
2018-10-08
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dc.description.abstract
It is necessary to determine the limit of detection when validating any analytical method. For methods with a linear response, a simple and low labor-consuming procedure is to use the linear regression parameters obtained in the calibration to estimate the blank standard deviation from the residual standard deviation (sres), or the intercept standard deviation (sb0). In this study, multiple experimental calibrations are evaluated, applying both ordinary and weighted least squares. Moreover, the analyses of replicated blank matrices, spiked at 2–5 times the lowest calculated limit values with the two regression methods, are performed to obtain the standard deviation of the blank. The limits of detection obtained with ordinary least squares, weighted least squares, the signal-to-noise ratio, and replicate blank measurements are then compared. Ordinary least squares, which is the simplest and most commonly applied calibration regression methodology, always overestimate the values of the standard deviations at the lower levels of calibration ranges. As a result, the detection limits are up to one order of magnitude greater than those obtained with the other approaches studied, which all gave similar limits
dc.description.sponsorship
This study has been supported by the University of Girona (MPCUdG2016/100)
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application/pdf
dc.language.iso
eng
dc.publisher
MDPI (Multidisciplinary Digital Publishing Institute)
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Reproducció digital del document publicat a: https://doi.org/10.3390/separations5040049
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Separations, 2018, vol. 5, núm. 4, p. 49
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Articles publicats (D-Q)
dc.rights
Attribution 4.0 International
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dc.subject
dc.title
Estimating Detection Limits in Chromatography from Calibration Data: Ordinary Least Squares Regression vs. Weighted Least Squares
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
2297-8739