Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
dc.contributor.author
dc.date.accessioned
2018-06-08T10:18:15Z
dc.date.available
2018-06-08T10:18:15Z
dc.date.issued
2017-09-14
dc.identifier.uri
dc.description.abstract
This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of cholesterol by confocal microscopy live-cell imaging. Subsequently, the effects of optimized CLC concentrations and incubation times prior to vitrification on early embryo development were assessed. Then, we evaluated the effects of pretreatment with 2 mg/ml CLC for 30 min before the vitrification of immature (GV) and in vitro matured (MII) oocytes on developmental competence and gene expression. Our results indicate a high plasma membrane labeling intensity after 30 min of incubation with 2 mg/ml CLC for 30 min, regardless of the holding medium used. When oocytes were incubated with 1 mg/ml, 2 mg/ml and 3 mg/ml of CLC, intense labeling was observed at the plasma membrane after 40, 30 and 20 min, respectively. CLC pre-treatment before the vitrification of bovine oocytes did not affect subsequent cleavage and embryo development rates irrespective of CLC concentrations, incubation times or meiotic stage. However, pretreatment seems to improve the quality of embryos derived from vitrified oocytes, mainly when oocytes were vitrified at the GV stage
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science (PLoS)
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Reproducció digital del document publicat a: https://doi.org/10.1371/journal.pone.0184714
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PLoS One, 2017, vol. 12, núm. 9, p. e0184714
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
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dc.source
Arcarons, Núria Morató, Roser Vendrell, Meritxell Yeste Oliveras, Marc López-Bejar, Manel Rajapaksha, Kosala Anzar, Muhammad Mogas, Teresa 2017 Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes PLoS One 12 9 e0184714
dc.title
Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
028520
dc.type.peerreviewed
peer-reviewed
dc.identifier.eissn
1932-6203