An Optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species
dc.contributor.author
dc.date.accessioned
2018-05-23T06:36:18Z
dc.date.available
2018-05-23T06:36:18Z
dc.date.issued
2017-09-01
dc.identifier.issn
1749-4869
dc.identifier.uri
dc.description.abstract
The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with males for DNA isolation. Using Aristeus antennatus as a model we tested a reliable non‐invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum. The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping. This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs. More importantly, it can be applied to identify the mating pairs and male structure in wild populations of species as A. antennatus, where males are often difficult to capture. Our method could be also valuable for biological studies on other spermatophore‐using species, such as myriapods, arachnids and insect
dc.description.sponsorship
This work was supported by a grant from Spanish Ministerio de Economía y Competitividad (CTM2014-54648-C2-2-R) to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.relation
info:eu-repo/grantAgreement/MINECO//CTM2014-54648-C2-2-R/ES/CONECTIVIDAD POBLACIONAL EN LA GAMBA ROSADA, ARISTEUS ANTENNATUS, EN EL MEDITERRANEO NOROCCIDENTAL ENTRE EL GOLFO DE LEON Y EL GOLFO DE VALENCIA.-FLUJO GENICO./
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1111/1749-4877.12250
dc.relation.ispartof
© Integratize zoology, 2017, vol. 12, núm. 5, p. 421-427
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
An Optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
026164
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.ProjectAcronym
dc.identifier.eissn
1749-4877