DUGiDocsAquaglyceroporins 3 and 7 in bull spermatozoa: Identification, localisation and their relationship with sperm cryotolerance
dc.contributor.author
dc.date.accessioned
2018-05-22T07:28:03Z
dc.date.available
2018-05-22T07:28:03Z
dc.date.issued
2017-01-01
dc.identifier.issn
1031-3613
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dc.description.abstract
The present study aimed to determine the localisation of aquaglyceroporins 3 (AQP3) and 7 (AQP7) in bull spermatozoa and their relationship with the sperm cell's resilience to withstand cryopreservation (i.e. cryotolerance). A total of 18 bull ejaculates were cryopreserved and their sperm quality analysed before and after freeze-thawing. The presence and localisation of AQP3 and AQP7 was determined through immunoblotting and immunocytochemistry. AQP3 was found in the mid-piece and AQP7 in the mid-piece and post-acrosomal region of bull spermatozoa. Immunoblotting showed specific signal bands at 30 and 60kDa for AQP3 and at 25kDa for AQP7. Neither the relative abundance of AQP3 and AQP7 nor their localisation patterns was altered by cryopreservation but individual differences between bull ejaculates were found in immunoblots. In order to determine whether these individual differences were related to sperm cryotolerance, bull ejaculates were classified as having good (GFE) or poor freezability (PFE) on the basis of their sperm quality after thawing. While the relative abundance of AQP3 before cryopreservation did not differ between ejaculates with GFE and PFE, the abundance of AQP7 was higher in GFE than in PFE ejaculates. This finding was further confirmed through principal component and linear regression analyses. In conclusion, the relative abundance of AQP7 in fresh semen may be used as a marker to predict bull sperm cryotolerance
dc.description.sponsorship
The authors acknowledge support from the Regional Government of Catalonia (Generalitat de Catalunya, Spain; Grant code: 2009-SGR-745) and the Ministry of Economy and Competitiveness, Spain (Grant code: AGL2013–47798-P). N. P.-M. was supported by a PhD Studentship funded by the Regional Government of Catalonia (Generalitat de Catalunya). R.M. was supported by a Postdoctoral Research Fellowship (‘Juan de la Cierva’ Scheme; Ministry of Economy and Competitiveness, Spain). M. Y. is funded by the European Commission, FP7-People Program, Marie Curie-IEF (Grant Number: 626061).
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application/pdf
dc.language.iso
eng
dc.publisher
CSIRO Publishing
dc.relation
info:eu-repo/grantAgreement/MINECO//AGL2013-47798-P/ES/EVALUACION DE LOS PEPTIDOS ANTIMICROBIANOS COMO SUSTITUTOS DE LOS ANTIBIOTICOS EN LOS DILUYENTES DE REFRIGERACION DEL SEMEN PORCINO/
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Reproducció digital del document publicat a: https://doi.org/10.1071/RD16077
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© Reproduction, Fertility and Development, 2017, vol. 29, núm. 6, p. 1249-1259
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Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Aquaglyceroporins 3 and 7 in bull spermatozoa: Identification, localisation and their relationship with sperm cryotolerance
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
025817
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.ProjectAcronym
dc.identifier.eissn
1448-5990