Electrospinning Parameters Selection to Manufacture Polycaprolactone Scaffolds for Three-dimensional Breast Cancer Cell Culture and Enrichment
dc.contributor.author
dc.date.accessioned
2018-02-20T14:58:55Z
dc.date.available
2018-02-20T14:58:55Z
dc.date.issued
2017
dc.identifier.issn
2212-8271
dc.identifier.uri
dc.description.abstract
Only a small cell population of a breast tumour presents stem cell characteristics, thus so-called breast cancer stem cells (BCSCs). BCSCs are tumour-initiating cells and chemoresistant and they can grow in non-adherent conditions as mammospheres. Study of BCSCs is a challenge due to their low representation and the inability to propagate them without inducing differentiation. Previous studies have demonstrated that three-dimensional (3D) cell culture models such as scaffolds enhance the BCSCs population.
In this project, scaffolds were fabricated by electrospinning technology. Specific values of voltage and flow rate were fixed to electrospun 7.5 and 15% poly(ɛ-caprolactone) (PCL) solutions according to process stability. Both scaffolds were seeded with MCF-7 breast cancer cells and 7.5% meshes displayed lower cell proliferation compared with 15% scaffolds. Cells cultured in both scaffolds presented a significant Mammosphere Forming Index (MFI) increase, thus indicating a BCSCs enrichment.
Results show that three-dimensional cell culture with electrospun 15% PCL scaffolds could be useful to expand BCSCs population facilitating the future development of new therapeutic strategies against this tumour subpopulation
dc.description.sponsorship
This work was funded partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III
(PI1400329) and by European Regional Development Fund (FEDER) and Ministerio de Economía y Competitividad
(DPI2013-45201-P) and the support of the Catalonian government (2014SGR00868)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
info:eu-repo/grantAgreement/MINECO//DPI2013-45201-P/ES/ESTUDIO Y DESARROLLO DE NUEVAS TECNOLOGIAS PARA SU APLICACION EN LA FABRICACION DE IMPLANTES/
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1016/j.procir.2017.03.341
dc.relation.ispartof
Procedia CIRP, 2017, vol. 65, p. 267-272
dc.relation.ispartofseries
Articles publicats (D-EMCI)
dc.rights
Attribution-NonCommercial-NoDerivs 4.0 Spain
dc.rights.uri
dc.subject
dc.title
Electrospinning Parameters Selection to Manufacture Polycaprolactone Scaffolds for Three-dimensional Breast Cancer Cell Culture and Enrichment
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
027878
dc.contributor.funder
dc.type.peerreviewed
peer-reviewed
dc.relation.ProjectAcronym