DUGiDocsValidated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
dc.contributor.author
dc.date.accessioned
2017-05-22T11:18:58Z
dc.date.available
2017-05-22T11:18:58Z
dc.date.issued
2016-12-14
dc.identifier.issn
2045-2322
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dc.description.abstract
The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations
dc.description.sponsorship
We are indebted to different colleagues for zebra mussel sample collections: Concha Durán (Ebro Hydrografic
Confederation, Spain) for the logistic advice; Anna Terrats and Carolina Solà (Catalan Water Agency from the
Government of Catalonia, Spain) for the filtered-water environmental samples. We would thank to Mariona
Palacios for her collaboration in the analysis into her Final Master Project. This research was carried out
within the objectives of the research project CGL200909407 of the Spanish Ministerio de Ciencia e Innovación
(MICINN)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation
MICINN/PN 2010-2013/CGL2009-09407
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Reproducció digital del document publicat a: http://dx.doi.org/10.1038/srep39067
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Scientific Reports, núm. 6, art. 39067
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Articles publicats (D-B)
dc.rights
Attribution 4.0 Spain
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dc.subject
dc.title
Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
025965
dc.contributor.funder