Does advancing male age influence the expression levels and localisation patterns of phospholipase C zeta (PLCζ) in human sperm?
dc.contributor.author
dc.date.accessioned
2017-05-22T10:35:13Z
dc.date.available
2017-05-22T10:35:13Z
dc.date.issued
2016-06-08
dc.identifier.issn
2045-2322
dc.identifier.uri
dc.description.abstract
Socio-economic factors have led to an increasing trend for couples to delay parenthood. However, advancing age exerts detrimental effects upon gametes which can have serious consequences upon embryo viability. While such effects are well documented for the oocyte, relatively little is known with regard to the sperm. One fundamental role of sperm is to activate the oocyte at fertilisation, a process initiated by phospholipase C zeta (PLCζ), a sperm-specific protein. While PLCζ deficiency can lead to oocyte activation deficiency and infertility, it is currently unknown whether the expression or function of PLCζ is compromised by advancing male age. Here, we evaluate sperm motility and the proportion of sperm expressing PLCζ in 71 males (22–54 years; 44 fertile controls and 27 infertile patients), along with total levels and localisation patterns of PLCζ within the sperm head. Three different statistical approaches were deployed with male age considered both as a categorical and a continuous factor. While progressive motility was negatively correlated with male age, all three statistical models concurred that no PLCζ–related parameter was associated with male age, suggesting that advancing male age is unlikely to cause problems in terms of the sperm’s fundamental ability to activate an oocyte
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1038/srep27543
dc.relation.ispartof
Scientific Reports, núm. 6, art. 27543
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Attribution 4.0 Spain
dc.rights.uri
dc.subject
dc.title
Does advancing male age influence the expression levels and localisation patterns of phospholipase C zeta (PLCζ) in human sperm?
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
025816