Diversity of Miscellaneous Crenarchaeotic Group archaea in freshwater karstic lakes and their segregation between planktonic and sediment habitats
dc.contributor.author
dc.date.accessioned
2017-01-13T11:12:27Z
dc.date.available
2017-01-13T11:12:27Z
dc.date.issued
2015-01
dc.identifier.issn
0168-6496
dc.identifier.uri
dc.description.abstract
The Miscellaneous Crenarchaeotic Group (MCG) is an archaeal lineage whose members are widespread and abundant in marine
sediments. MCG archaea have also been consistently found in stratified euxinic lakes. In this work, we have studied
archaeal communities in three karstic lakes to reveal potential habitat segregation of MCG subgroups between planktonic
and sediment compartments. In the studied lakes, archaeal assemblages were strikingly similar to those of the marine
subsurface with predominance of uncultured Halobacteria in the plankton and Thermoplasmata and MCG in anoxic,
organic-rich sediments. Multivariate analyses identified sulphide and dissolved organic carbon as predictor variables of
archaeal community composition. Quantification of MCG using a newly designed qPCR primer pair that improves coverage
for MCG subgroups prevalent in the studied lakes revealed conspicuous populations in both the plankton and the sediment.
Subgroups MCG-5a and -5b appear as planktonic specialists thriving in euxinic bottom waters, while subgroup MCG-6
emerges as a generalist group able to cope with varying reducing conditions. Besides, comparison of DNA- and cDNA-based
pyrotag libraries revealed that rare subgroups in DNA libraries, i.e. MCG-15, were prevalent in cDNA-based datasets,
suggesting that euxinic, organic-rich sediments of karstic lakes provide optimal niches for the activity of some specialized
MCG subgroups
dc.description.sponsorship
This research was funded by projects ARCANOX (Ref. CGL2009-13318-C02–02) and ARCOS (ref. CGL2012–33033) from the Spanish Ministry of Economy and Competitiveness (MINECO)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.relation
MICINN/PN 2010-2012/CGL2009-13318-C02-02
info:eu-repo/grantAgreement/MINECO//CGL2012-33033/ES/CONTRIBUCION DE LAS ARCHAEA NO CULTIVADAS EN EL RECICLAJE DE CARBONO ORGANICO EN SEDIMENTOS/
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Reproducció digital del document publicat a: http://dx.doi.org/10.1093/femsec/fiv020
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© FEMS Microbiology Ecology, 2015, vol. 91, p. fiv020
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Articles publicats (D-B)
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Tots els drets reservats
dc.subject
dc.title
Diversity of Miscellaneous Crenarchaeotic Group archaea in freshwater karstic lakes and their segregation between planktonic and sediment habitats
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
023887
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1574-6941