Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
dc.contributor.author
dc.date.accessioned
2016-10-27T10:59:07Z
dc.date.available
2016-10-27T10:59:07Z
dc.date.issued
2016
dc.identifier.uri
dc.description.abstract
The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60° scaffolds were more suitable for 3D culture than the 45° and 90° ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs population
dc.description.sponsorship
This work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
MDPI (Multidisciplinary Digital Publishing Institute)
dc.relation
info:eu-repo/grantAgreement/MINECO//DPI2013-45201-P/ES/ESTUDIO Y DESARROLLO DE NUEVAS TECNOLOGIAS PARA SU APLICACION EN LA FABRICACION DE IMPLANTES/
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.3390/molecules21040537
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Molecules, 2016, vol. 21, núm. 4, p. 537
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Articles publicats (D-CM)
dc.rights
Attribution 4.0 Spain
dc.rights.uri
dc.subject
dc.title
Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
025307
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dc.relation.ProjectAcronym
dc.identifier.eissn
1420-3049