Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus.
dc.contributor.author
dc.date.accessioned
2016-07-29T09:55:09Z
dc.date.available
2016-07-29T09:55:09Z
dc.date.issued
2016-07-27
dc.identifier.uri
dc.description.abstract
The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource
dc.format.extent
14 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.relation
info:eu-repo/grantAgreement/MICINN//AGL2009-09228/ES/Gambas Rojas: Filogeografia, Identificacion Genetica De Los Estocs Pesqueros Mediante Analisis De Adn E Implicancias Para Su Gestion./
info:eu-repo/grantAgreement/MINECO//CTM2014-54648-C2-2-R/ES/CONECTIVIDAD POBLACIONAL EN LA GAMBA ROSADA, ARISTEUS ANTENNATUS, EN EL MEDITERRANEO NOROCCIDENTAL ENTRE EL GOLFO DE LEON Y EL GOLFO DE VALENCIA.-FLUJO GENICO./
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.7717/peerj.2200
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PeerJ, 2016, vol. e2200, p. 1-14
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Articles publicats (D-B)
dc.rights
Reconeixement 4.0 Internacional
dc.rights.uri
dc.source
Heras Mena, Sandra Planella, Laia Caldarazzo, Ilaria Vera Rodríguez, Manuel García Marín, José Luis Roldán Borassi, María Inés 2016 Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus. PeerJ e2200 1 14
dc.subject
dc.title
Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus.
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
025448
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
2167-8359