Effect of Pseudomonas aeruginosa on sperm capacitation and protein phosphorylation of boar spermatozoa
dc.contributor.author
dc.date.accessioned
2016-07-26T08:22:31Z
dc.date.available
2016-07-26T08:22:31Z
dc.date.issued
2016
dc.identifier.issn
0093-691X
dc.identifier.uri
dc.description.abstract
Several studies have reported the detrimental effects that bacteriospermia causes on boar sperm quality, but little is known about its effects on IVC. Considering that, the present study sought to evaluate the effects of different concentrations of Pseudomonas aeruginosa on different indicators of capacitation status (sperm viability, membrane lipid disorder, sperm motility kinematics, and protein phosphorylation of boar spermatozoa) after IVC. Flow cytometry and computer assisted sperm analysis (CASA) revealed that the presence of P aeruginosa in boar sperm samples, mostly at concentrations greater than 106 CFU/mL, is associated with a significant (P < 0.05) decrease in the percentages of both sperm membrane integrity and sperm with low membrane lipid disorder, and also with a reduction in sperm motility kinetic parameters when compared with results obtained from the control sample, which presented the typical motility pattern of capacitated-like boar spermatozoa. Moreover, Western blot results also showed significant (P < 0.05) changes in the levels of tyrosine, serine, and threonine protein phosphorylation because of bacterial contamination, the decrease in phosphotyrosine levels of p32, a well-known marker of IVC achievement in boar sperm, being the most relevant. Indeed, after 3 hours of IVC, phosphotyrosine levels of p32 in the control sample were 3.13 ± 0.81, whereas in the tubes with 106 and 108 CFU/mL were 1.05 ± 0.20 and 0.36 ± 0.07, respectively. Therefore, the present study provides novel data regarding the effects of bacterial contamination on boar sperm, suggesting that the presence of P aeruginosa affects the fertilizing ability of boar sperm by altering its ability to accomplish IVC
dc.description.sponsorship
This research was funded by Project RZ2011-0001-00-00 and AGL2013-47798P (INIA and Ministerio de Ciencia e Innovación, Spanish Government). Lilian Sepúlveda was supported by a predoctoral grant (FI-DGR 2013) from the Regional Government of Catalonia (Generalitat de Catalunya)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
info:eu-repo/grantAgreement/MINECO//AGL2013-47798-P/ES/EVALUACION DE LOS PEPTIDOS ANTIMICROBIANOS COMO SUSTITUTOS DE LOS ANTIBIOTICOS EN LOS DILUYENTES DE REFRIGERACION DEL SEMEN PORCINO/
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.theriogenology.2015.12.025
dc.relation.ispartof
© Theriogenology, 2015, vol. 85, núm. 8, 1421-1431
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Effect of Pseudomonas aeruginosa on sperm capacitation and protein phosphorylation of boar spermatozoa
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1879-3231