Changes in microbial biofilm communities during colonization of sewer systems
dc.contributor.author
dc.date.accessioned
2016-07-11T10:24:36Z
dc.date.available
2016-07-11T10:24:36Z
dc.date.issued
2015-08-07
dc.identifier.issn
0099-2240
dc.identifier.uri
dc.description.abstract
The coexistence of sulfate-reducing bacteria (SRB) and methanogenic archaea (MA) in anaerobic biofilms developed in sewer inner pipe surfaces favors the accumulation of sulfide (H2S) and methane (CH4) as metabolic end products, causing severe impacts on sewerage systems. In this study, we investigated the time course of H2S and CH4 production and emission rates during different stages of biofilm development in relation to changes in the composition of microbial biofilm communities. The study was carried out in a laboratory sewer pilot plant that mimics a full-scale anaerobic rising sewer using a combination of process data and molecular techniques (e.g., quantitative PCR [qPCR], denaturing gradient gel electrophoresis [DGGE], and 16S rRNA gene pyrotag sequencing). After 2 weeks of biofilm growth, H2S emission was notably high (290.7 ± 72.3 mg S-H2S liter-1 day-1), whereas emissions of CH4 remained low (17.9 ± 15.9 mg COD-CH4 liter-1 day-1). This contrasting trend coincided with a stable SRB community and an archaeal community composed solely of methanogens derived from the human gut (i.e., Methanobrevibacter and Methanosphaera). In turn, CH4 emissions increased after 1 year of biofilm growth (327.6 ± 16.6 mg COD-CH4 liter-1 day-1), coinciding with the replacement of methanogenic colonizers by species more adapted to sewer conditions (i.e., Methanosaeta spp.). Our study provides data that confirm the capacity of our laboratory experimental system to mimic the functioning of full-scale sewers both microbiologically and operationally in terms of sulfide and methane production, gaining insight into the complex dynamics of key microbial groups during biofilm development
dc.description.sponsorship
Authors thank the four anonymous reviewers for the useful comments and suggestions made during the review process. This study was partially funded by the Spanish Government Ministerio de Economía y Competitividad through projects GEISTTAR (CTM2011-27163) and ARCOS (CGL2012-33033) and by the European Commission through projects FP7-PEOPLE-2011-CIG303946, 2010-RG277050 and the ITN-Project SANITAS (the REA agreement 289193). Maite Pijuan is a recipient of a Ramon y Cajal research fellowship (RYC-2009-04959) and Olga Auguet benefits from a FI research fellowship (2014FI_B1 00032) from the Catalan Government. ICRA is a recipient of a Consolidated Research Group grant (2014 SGR 291) from the Catalan Gove
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.relation
info:eu-repo/grantAgreement/MICINN//CTM2011-27163/ES/GASES DE EFECTO INVERNADERO EN LOS SISTEMAS DE TRANSPORTE Y TRATAMIENTO DE AGUAS RESIDUALES: EVALUACION DE LAS EMISIONES/
info:eu-repo/grantAgreement/MINECO//CGL2012-33033/ES/CONTRIBUCION DE LAS ARCHAEA NO CULTIVADAS EN EL RECICLAJE DE CARBONO ORGANICO EN SEDIMENTOS/
info:eu-repo/grantAgreement/MICINN//RYC-2009-04959/ES/RYC-2009-04959/
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1128/AEM.01538-15
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© Applied and Environmental Microbiology, 2015, vol. 81, núm. 20, p. 7271-7280
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Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Changes in microbial biofilm communities during colonization of sewer systems
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.relation.projectID
info:eu-repo/grantAgreement/EC/FP7/303946/EU/Exploring novel nitrifier pathways to minimise direct greenhouse gas emissions from WWTPs./NITRI-GHG
info:eu-repo/grantAgreement/EC/FP7/277050/EU/SULFIDE AND GREENHOUSE GAS EMISIONS FROM MEDITERRANEAN SEWERS/SGHGEMS
info:eu-repo/grantAgreement/EC/FP7/289193/EU/Sustainable and integrated urban water system management/SANITAS
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
024866
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1098-5336