A PCR technique to detect enterotoxigenic and verotoxigenic Escherichia coli in boar semen samples
dc.contributor.author
dc.date.accessioned
2016-05-05T10:12:48Z
dc.date.available
2016-05-05T10:12:48Z
dc.date.issued
2012
dc.identifier.issn
0034-5288
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dc.description.abstract
In semen, bacteria's isolation from a pure culture is complex, laborious and easily alterable by the presence of antibiotics and inhibitors. We developed a PCR technique to detect the presence of the enterotoxigenic (ETEC) and verotoxigenic Escherichia coli (VTEC) (strains with high prevalence in the swine industry) in semen by adapting the protocols developed by Zhang et al. (2007) and Yilmaz et al. (2006). We artificially inoculated extended semen samples at different infective concentrations of bacteria (from 10 2 to 10 8 bacteriaml -1) with two enterotoxigenic and verotoxigenic strains, and performed two multiplex and one conventional PCR. This technique proved to be a quick, useful and reliable tool to detect the presence of ETEC and VTEC up to an infective dose of 10 5 bacteriaml -1 in semen
dc.description.sponsorship
This research was supported by Project RZ2008-0001-00-00, funds from the ‘‘Ministerio de Ciencia e Innovación’’ (Spanish Government)
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application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
MICINN/PN 2011-2014/RZ2011-00001-00-00
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Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.rvsc.2011.07.012
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© Research in Veterinary Science, 2012, vol. 93, núm. 1, p. 31-33
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Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
A PCR technique to detect enterotoxigenic and verotoxigenic Escherichia coli in boar semen samples
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
021385
dc.contributor.funder
dc.identifier.eissn
1532-2661