DUGiDocs1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen
dc.contributor.author
dc.date.accessioned
2016-01-28T06:51:58Z
dc.date.available
2016-01-28T06:51:58Z
dc.date.issued
2013
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1874-2718
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dc.description.abstract
Onconase® FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase® sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase's® active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR 1H, 13C and 15N resonance chemical shift assignments at pH 5.2 and 35 C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase ® is conserved in the FL-G zymogen
dc.description.sponsorship
This work was supported by the projects, CTQ2008-0080, CTQ2010-21567-C02-02 and BFU2009-06935/ BMC from MICINN and PUG2008A from the Universitat de Girona
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application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.relation
info:eu-repo/grantAgreement/MICINN//BFU2009-06935/ES/Bases Moleculares Del Plegamiento Y Citotoxicidad De Las Ribonucleasas Pancreaticas. Evaluacion De La Actividad Citotoxica Y Diseño De Estrategias Para Su Control Mediante Splicing Proteico./
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Reproducció digital del document publicat a: http://dx.doi.org/10.1007/s12104-012-9367-0
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© Biomolecular NMR Assignments, 2013, vol. 7, p. 13-15
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Articles publicats (D-B)
dc.rights
Tots els drets reservats
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dc.title
1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen
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info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
017120
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dc.relation.ProjectAcronym
dc.identifier.eissn
1874-270X