Production of biologically active Cecropin A peptide in rice seed oil bodies
dc.contributor.author
dc.date.accessioned
2016-01-18T09:02:41Z
dc.date.available
2016-01-18T09:02:41Z
dc.date.issued
2016-01-13
dc.identifier.uri
dc.description.abstract
Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production and purification costs. Here, we report the efficient production of this bioactive peptide in rice bran using the rice oleosin 18 as a carrier protein. High cecropin A levels were reached in rice seeds driving the expression of the chimeric gene by the strong embryo-specific oleosin 18 own promoter, and targeting the peptide to the oil body organelle as an oleosin 18-cecropin A fusion protein. The accumulation of cecropin A in oil bodies had no deleterious effects on seed viability and seedling growth, as well as on seed yield. We also show that biologically active cecropin A can be easily purified from the transgenic rice seeds by homogenization and simple flotation centrifugation methods. Our results demonstrate that the oleosin fusion technology is suitable for the production of cecropin A in rice seeds, which can potentially be extended to other antimicrobial peptides to assist their exploitation
dc.description.sponsorship
This work was supported by the Spanish Ministry of Economy and Competitiveness [Grants EUI2008-03769, EUI2008-03572 and BIO2012- 32838], and by the French Research Agency [Grant ANR-08-KBBE-010]
dc.format.extent
18 p.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science (PLoS)
dc.relation
info:eu-repo/grantAgreement/MICINN//EUI2008-03572/ES/PLANTAS BIOFACTORIA DE PEPTIDOS ANTIMICROBIANOS/
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Reproducció digital del document publicat a: http://dx.doi.org/10.1371/journal.pone.0146919
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PLoS One, 2016, vol. 11, num. 1, p. e0146919-e0146937
dc.rights
Reconeixement 3.0 Espanya
dc.rights.uri
dc.source
Montesinos Barreda, Laura Bundó, Mireia Izquierdo, Esther Campo, Sonia Badosa Romañó, Esther Rossignol, Michel Montesinos Seguí, Emilio San Segundo de los Mozos, Blanca Coca, María 2016 Production of biologically active Cecropin A peptide in rice seed oil bodies PLoS One 11 1 e0146919 e0146937
dc.subject
dc.title
Production of biologically active Cecropin A peptide in rice seed oil bodies
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
024186
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1932-6203
1932-6203