Mucosa-associated Faecalibacterium prausnitzii phylotype richness is reduced in patients with inflammatory bowel disease
dc.contributor.author
dc.date.accessioned
2015-12-09T12:52:45Z
dc.date.available
2016-05-01T03:00:06Z
dc.date.issued
2015
dc.identifier.issn
0099-2240
dc.identifier.uri
dc.description
El Títol de la versió postprint o Accepted Manuscript de l'article és 'Mucosa-associated Faecalibacterium prausnitzii phylotype richness is reduced in inflammatory bowel disease patients'
dc.description.abstract
Faecalibacterium prausnitzii depletion in intestinal diseases has been extensively reported, but little is known about intraspecies variability. This work aims to determine if subjects with gastrointestinal disease host mucosa-associated F. prausnitzii populations different from those hosted by healthy individuals. A new species-specific PCR-denaturing gradient gel electrophoresis (PCR-DGGE) method targeting the 16S rRNA gene was developed to fingerprint F. prausnitzii populations in biopsy specimens from 31 healthy control (H) subjects and 36 Crohn's disease (CD), 23 ulcerative colitis (UC), 6 irritable bowel syndrome (IBS), and 22 colorectal cancer (CRC) patients. The richness of F. prausnitzii subtypes was lower in inflammatory bowel disease (IBD) patients than in H subjects. The most prevalent operational taxonomic units (OTUs) consisted of four phylotypes (OTUs with a 99% 16S rRNA gene sequence similarity [OTU99]), which were shared by all groups of patients. Their distribution and the presence of some disease-specific F. prausnitzii phylotypes allowed us to differentiate the populations in IBD and CRC patients from that in H subjects. At the level of a minimum similarity of 97% (OTU97), two phylogroups accounted for 98% of the sequences. Phylogroup I was found in 87% of H subjects but in under 50% of IBD patients (P = 0.003). In contrast, phylogroup II was detected in >75% of IBD patients and in only 52% of H subjects (P = 0.005). This study reveals that even though the main members of the F. prausnitzii population are present in both H subjects and individuals with gut diseases, richness is reduced in the latter and an altered phylotype distribution exists between diseases. This approach may serve as a basis for addressing the suitability of F. prausnitzii phylotypes to be quantified as a putative biomarker of disease and depicting the importance of the loss of these subtypes in disease pathogenesis
dc.description.sponsorship
This work was partially funded by the Spanish Ministry of Education and Science through project SAF2010-15896. Mireia Lopez-Siles was the recipient of an FI grant from the Generalitat de Catalunya (2010FI_B2 00135), which receives support from the European Union Commission. Harry J. Flint and Sylvia H. Duncan acknowledge support from the Food, Land, and People program of the Scottish government
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.relation
info:eu-repo/grantAgreement/MICINN//SAF2010-15896/ES/ESCHERICHIA COLI ADHERENTE INVASIVA (AIEC): PREVALENCIA EN OTRAS ENFERMEDADES INTESTINALES DISTINTAS DE CROHN Y DETERMINANTES GENICOS IMPLICADOS EN SU PATOGENICIDAD./
dc.relation.isformatof
Versió postprint del document publicat a: http://dx.doi.org/10.1128/AEM.02006-15
dc.relation.ispartof
© Applied and Environmental Microbiology, 2015, vol. 81, núm. 21, p. 7582-7592
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Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Mucosa-associated Faecalibacterium prausnitzii phylotype richness is reduced in patients with inflammatory bowel disease
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/acceptedVersion
dc.identifier.doi
dc.identifier.idgrec
024436
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1098-5336